Read more
This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.
List of contents
The Importance of Experimental Design, Quality Assurance, and Control in Plant Metabolomics Experiments.- Standard Key Steps in Mass Spectrometry-Based Plant Metabolomics Experiments: Instrument Performance and Analytical Method Validation.- Guidelines for Sample Normalization to Minimize Batch Variation for Large-Scale Metabolic Profiling of Plant Natural Genetic Variance.- Gas Chromatography-Mass Spectrometry-Based 13C-Labeling Studies in Plant Metabolomics.- Carbon Atomic Survey for Identification of Selected Metabolic Fluxes.- Quantification of Low-Abundant Phosphorylated Carbohydrates Using HILIC-QqQ-MS/MS.- Quantification of Soluble Sugars and Sugar Alcohols by LC-MS/MS.- Untargeted Analysis of Semi-Polar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation.- Extraction of Plant Lipids for LC-MS-Based Untargeted Plant Lipidomics.- Semi-Targeted Lipidomics of Plant Acyl Lipids Using UPLC-HR-MS in Combination with a Data-Independent Acquisition Mode.- Plant Lipidomics Using UPLC-QTOF-MS.- Targeted LC-MS Analysis for Plant Secondary Metabolites.- An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones.- A Complete Pipeline for Generating a High-Resolution LC-MS-Based Reference Mass Spectra Library.- Quantification of Sugars and Organic Acids in Biological Matrices Using GC-QqQ-MS.- Acquisition of Volatile Compounds by Gas Chromatography-Mass Spectrometry (GC-MS).- Mass Spectrometry Imaging (MSI) for Plant Metabolomics.- Laser Ablation Electrospray Ionization-Mass Spectrometry Imaging (LAESI-MS) for Spatially Resolved Plant Metabolomics.- Live Single-Cell Mass Spectrometry (LSC-MS) for Plant Metabolomics.- Statistical and Multivariate Analysis of MS-Based Plant Metabolomics Data.- Integration of Plant Metabolomics Data into Metabolic Networks: Progresses and Challenges.- Understanding the Functionality of a Biological System as a Whole: Comparative Data Analysis.- Regression-Based Modeling of Complex Plant Traits Based on Metabolomics Data.- Mathematical Modeling Approaches in Plant Metabolomics.
Summary
This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.
