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This volume provides protocols for the generation of various biomaterials for tissue engineering and regenerative medicine applications. The chapters in this book include a look at a range of biomaterials including hydrogels and other matrices (natural, synthetic, self-healing) for various applications including drug and gene delivery, surface modification and functionalization of biomaterials. In addition, techniques described include those for controlling biomaterial geometry, such as three-dimensional printing and electrospinning. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Biomaterials for Tissue Engineering: Methods and Protocols is a valuable resource for scientists and engineers interested in this vital field of study.
List of contents
Engineering Citric-Acid Based Porous Scaffolds for Bone Regeneration.- Multifunctional Self-Assembling Peptide-Based Micelles for Targeted Intracellular Delivery: Design, Physicochemical Characterization, and Biological Assessment.- Electrospinning Functionalized Polymers for Use as Tissue Engineering Scaffolds.- Low-Temperature Deposition Modelling for CP Scaffolds with Controlled Bimodal Porosity.- Three-Dimensional Hydrogel-Based Culture to Study the Effects of Toxicants on Ovarian Follicles.- Layer-by-Layer Engineered Polymer Capsules for Therapeutic Delivery.- Controlling Fibrin Network Morphology, Polymerization, and Degradation Dynamics in Fibrin Gels for Promoting Tissue Repair.- Biofunctionalization of Poly(acrylamide) Gels.- Synthetic PEG Hydrogel for Engineering the Environment of Ovarian Follicles.- Engineering Human Neural Tissue by 3D Bioprinting.- High-Throughput Formation of Mesenchymal Stem Cell Spheroids and Entrapment in Aliginate Hydrogels.- Crimped Electrospun Fibers for Tissue Engineering.- In Vitro Model of Macrophage-Biomaterial Interactions.- Synthesis of Self-Assembling Peptide-Based Hydrogels for Regenerative Medicine Using Solid-Phase Peptide Synthesis.- H2S Delivery from Aromatic Peptide Amphiphile Hydrogels.
Summary
This volume provides protocols for the generation of various biomaterials for tissue engineering and regenerative medicine applications. The chapters in this book include a look at a range of biomaterials including hydrogels and other matrices (natural, synthetic, self-healing) for various applications including drug and gene delivery, surface modification and functionalization of biomaterials. In addition, techniques described include those for controlling biomaterial geometry, such as three-dimensional printing and electrospinning. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Biomaterials for Tissue Engineering: Methods and Protocols is a valuable resource for scientists and engineers interested in this vital field of study.
