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This volume details protocols that broadly cover many aspects of basic and translational research on Borrelia burgdorferi. Chapters guide readers through epidemiology and ecology, cultivation, cell structure, physiology, genomics and transcriptomics, proteomics, animal infection, pathogenesis and host responses, and vaccines. These essential protocols incorporate the most recent, practical, and innovative research tools aiding new and experienced researchers in their studies involving the biology, pathogenesis, and prevention of B. burgdorferi infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Borrelia burgdorferi:Methods and Protocols aims to ensure successful results in the further study of this vital field of biomedical research.
List of contents
Genotyping Strains of Lyme Disease Agents Directly From Ticks, Blood, or Tissue.- Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato using Molecular Biological Methods.- Generation of Mammalian Host-adapted Borrelia burgdorferi by Cultivation in Peritoneal Dialysis Membrane Chamber Implantation in Rats.- Co-immunoprecipitation for Identifying Protein-protein Interactions in Borrelia Burgdorferi.- Characterization of Borrelia burgdorferi Binding to Mammalian Cells and Extracellular Matrix.- Analysis of Lipids and Lipid Rafts in Borrelia.- Trace Element AnalysisofBorrelia burgdorferi by Inductively Coupled Plasma-Sector Field Mass Spectrometry.- Identification and Characterization of Borrelia burgdorferiComplement-binding Proteins.- Artificial Infection of Ticks with Borrelia burgdorferi using a Microinjection Method and their Detection in vivo using Quantitative PCR Targeting flaB RNA.- Borrelia burgdorferi Microarray Analysis.- Borrelia burgdorferi Transcriptome Analysis by RNA-sequencing.- Selection of Borrelia burgdorferi Promoter Sequences Active during Mammalian Infection Using In Vivo Expression Technology.- Analysis of DNA and RNA Binding Properties of Borrelia burgdorferi Regulatory Proteins.- Identification of Acetylated Proteins in Borrelia burgdorferi.- Genetic Transformation and Complementation.- Genome-wide Mutagenesis in Borrelia burgdorferi.- Generation of Conditional Mutants in Borrelia burgdorferi.- Detection of Bioluminescent Borrelia burgdorferi from in vitro Cultivation and During Murine Infection.- Analysis of Borrelia burgdorferi Proteome and Protein-protein Interactions.- Two Photon Intravital Microscopy of Lyme Borrelia in Mice.- Investigating Human Dendritic Cell Immune Responses to Borrelia burgdorferi.- Phagocytosis Assays for Borrelia burgdorferi.- Measuring Borrelia burgdorferi Motility and Chemotaxis.- In vitro models of cutaneous inflammation.- Magnetic Isolation of Phagosomes Containing Toll like Receptor Ligands.- Xenodiagnosis using Ixodes scapularis Larval Ticks in Humans.- Recombinant E. coli Dualistic Role as an Antigen-adjuvant Delivery Vehicle for Oral Immunization.
Summary
This volume details protocols that broadly cover many aspects of basic and translational research on Borrelia burgdorferi. Chapters guide readers through epidemiology and ecology, cultivation, cell structure, physiology, genomics and transcriptomics, proteomics, animal infection, pathogenesis and host responses, and vaccines. These essential protocols incorporate the most recent, practical, and innovative research tools aiding new and experienced researchers in their studies involving the biology, pathogenesis, and prevention of B. burgdorferi infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Borrelia burgdorferi:Methods and Protocols aims to ensure successful results in the further study of this vital field of biomedical research.
