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This volume provides a comprehensive study of this key method of gel-based proteomics. Chapters in Difference Gel Electrophoresis: Methods and Protocols detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application of basic biological research, and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
List of contents
Two Dimensional Gel Electrophoresis and 2D-DIGE.- Comparative DIGE Proteomics.- 2D-DIGE and Fluorescence Image Analysis.- DIGE Analysis Software and Protein Identification Approaches.- Native DIGE: Efficient Tool to Elucidate Protein Interactomes.- Comparative Two-dimensional Fluorescence Gel Electrophoresis.- DIGE-based Phosphoproteomic Analysis.- DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-fixed Paraffin-embedded (FFPE) Tissue.- Comparative 3-sample DIGE Analysis of Skeletal Muscles.- DIGE Analysis of ProteoMiner(TM) Fractionated Serum/Plasma Samples.- DIGE Analysis of Human Tissues.- DIGE Analysis of Animal Tissues.- Rapid 2D DIGE Proteomic Analysis of Mouse Liver.- Proteomic Analysis of Lung Tissue by DIGE.- Comparative Testis Tissue Proteomics using 2-dye versus 3-dye DIGE Analysis.- DIGE Analysis of Fish Tissues.- Protein Digestion for DIGE Analysis.- Subcellular Fractionation for DIGE-based Proteomics.- DIGE Analysis of Immunodepleted Plasma.- Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments using Enzyme Assays.- Enzyme Assay Methods to Validate DIGE Proteomics Data.- Immunoblot Analysis of DIGE-based Proteomics.- Immunofluorescence Microscopy for DIGE-based Proteomics.
Summary
This volume provides a comprehensive study of this key method of gel-based proteomics. Chapters in Difference Gel Electrophoresis: Methods and Protocols detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application of basic biological research, and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
