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This detailed volume introduces the reader to current methods in glaucoma research, in particular those that make use of animal disease models. Given that the retinal ganglion cells damaged and destroyed by glaucoma do not regenerate after lowering the intraocular pressure, new therapeutic approaches that protect ganglion cells directly is a vital research goal that this volume's contents aim to aid scientists in developing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Glaucoma: Methods and Protocols serves as a useful guide for both ophthalmologists in clinical practice and researchers in the field.
List of contents
Use of Animal Models and Techniques in Glaucoma Research: Introduction.- Hypertonic Saline Injection Model of Experimental Glaucoma in Rats.- The Microbead Occlusion Model of Ocular Hypertension in Mice.- Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods.- Non-Invasive Intraocular Pressure Measurement in Animals Models of Glaucoma.- High Throughput Binocular Pattern Electroretinograms in the Mouse.- Visual Evoked Potentials in Glaucoma and Alzheimer's Disease.- Investigation of the Functional Retinal Output Using Microelectrode Arrays.- Quantitative Proteomic Analysis of Human Aqueous Humor Using iTRAQ 4plex Labeling.- Sphingolipid Analysis of Aqueous Humor.- Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion.- Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models.- Quantification of Scleral Biomechanics and Collagen Fiber Alignment.- Biolistic Labeling of RetinalGanglion Cells.- Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma.- In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration.- 3D Histomorphometric Reconstruction and Quantification of the Optic Nerve Head Connective Tissues.- Visualizing Astrocytes of the Optic Nerve.- Investigation of MicroRNA Expression in Experimental Glaucoma.- Utilizing RNA-Seq to Identify Differentially Expressed Genes in Glaucoma Model Tissues, Such as the Rodent Optic Nerve Head.- Single-Cell Dissociation and Characterization in the Murine Retina and Optic Nerve.
Summary
This detailed volume introduces the reader to current methods in glaucoma research, in particular those that make use of animal disease models. Given that the retinal ganglion cells damaged and destroyed by glaucoma do not regenerate after lowering the intraocular pressure, new therapeutic approaches that protect ganglion cells directly is a vital research goal that this volume’s contents aim to aid scientists in developing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Glaucoma: Methods and Protocols serves as a useful guide for both ophthalmologists in clinical practice and researchers in the field.
