Cellular Imaging - Electron Tomography and Related Techniques

This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging).
The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation

List of contents

TEM Tomography.- STEM Tomography.- CryoTomo.- Large Volume Electron Tomography.- Block Face Tomography.- Array Tomography.- Image Alignment.- Reconstruction Algorithms.- Subtomogram Averaging.- Resolution.- Signal Optimisation.- Segmentation.- Placing Macromolecular Complexes in Context.

Summary

This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). 
The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation

Product details

• Assisted by: Eri Hanssen (Editor), Eric Hanssen (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 01.01.2019
• EAN: 9783319886992
• ISBN: 978-3-31-988699-2
• No. of pages: 323
• Dimensions: 156 mm x 235 mm x 20 mm
• Weight: 534 g
• Illustrations: XIX, 323 p. 84 illus., 59 illus. in color.
• Series: Biological and Medical Physics, Biomedical Engineering; Biological and Medical Physics, Biomedical Engineering
• Subjects: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics; B, Microscopy, spectroscopy, Spectroscopy and Microscopy, Physics and Astronomy, Spectrum analysis, spectrochemistry, mass spectrometry, Scientific equipment, experiments & techniques, Spectroscopy/Spectrometry, Medical physics, Biophysics, Biological physics, Biological and Medical Physics, Biophysics, Biomedical Engineering and Bioengineering, Biomedical engineering