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This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Candida Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.
List of contents
How to use the Candida Genome Database.- Transcriptional Profiling of Candida albicans in the Host.- SRYTH: A New Yeast Two-Hybrid Method.- Chromatin immunoprecipitation (ChIP) assay in Candida albicans .- Assessing mitochondrial functions in Candida albicans.- Imaging Candida infections in the host.- Identification of Secreted Candida Proteins using Mass Spectrometry.- In vitro models for Candida Biofilm Development.- The Fungal Biome of the Oral Cavity.- Experimental models of C. albicans-streptococcal Co-infection.- Staphylococcus-Candida interaction models: Antibiotic Resistance Testing and Host Interaction.- Genetic Screens for Determination of Mechanism of Action.- Microbroth Dilution Susceptibility Testing of Candida Species.- Biofilms and Antifungal Susceptibility Testing.- Typing Candida Species using Microsatellite Length Polymorphism and Multilocus Sequence Typing.- Diagnostic Methods for Detection of Blood-borne Candidiasis.
Summary
This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Candida Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.
