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This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Protocols captures basic experimental protocols that are commonly used to study plant cell division processes, as well as more innovative procedures. Chapters are split into five parts covering several different aspect of plant cell division such as, cell cultures for cell division studies, cell cycle progression and mitosis, imaging plant cell division, cell division and morphogenesis, and cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Cell Division: Methods and Protocols is a valuable tool for the study of plant cell division at both the cellular and molecular levels, and in the context of plant development.
List of contents
Synchronization Of Green Algae By Light And Dark Regimes For Cell Cycle and Cell Division Studies.- Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells.- Identifying Gene Regulatory Networks In Arabidopsis By In Silico Prediction, Yeast-1-Hybrid and Inducible Gene Profiling Assays.- Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis.- Imaging Nuclear Morphology and Organization in Cleared Plant Tissues Treated with Cell Cycle Inhibitors.- Isolation of Plant Nuclei at Defined Cell Cycle Stages Using Edu Labeling and Flow Cytometry.- Preparation and Fluorescent Analysis of Plant Metaphase Chromosomes.- Fluorescence In Situ Hybridization On Rice Chromosomes.- Dynamics of the Plant Nuclear Envelope During Cell Division.- Immunolabeling Of Nuclei/Chromosomes In Arabidopsis Thaliana.- In Vivo Imaging Of Microtubules Organization In Dividing Giant Cell.- Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot Meristem.- Cell Proliferation Analysis Using Edu Labeling In Whole Plant and Histological Samples Of Arabidopsis.- Studying Cell Division Plane Positioning in Early-Stage Embryos.- Characterization Oof Cytokinetic Mutants using Small Fluorescent Probes.- Using Live Cell Markers in Maize to Analyze Cell Division Orientation and Timing.- Ratiometric Fluorescence Live Imaging Analysis of Membrane Lipid Order in Arabidopsis Mitotic Cells Using a Lipid Order-Sensitive Probe.
Summary
This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Protocols captures basic experimental protocols that are commonly used to study plant cell division processes, as well as more innovative procedures. Chapters are split into five parts covering several different aspect of plant cell division such as, cell cultures for cell division studies, cell cycle progression and mitosis, imaging plant cell division, cell division and morphogenesis, and cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Cell Division: Methods and Protocols is a valuable tool for the study of plant cell division at both the cellular and molecular levels, and in the context of plant development.
