Practical Guide to Protein and Peptide Purification for Microsequencin

Provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" This work includes methods such as: ways of dealing with blocked N termini, computer analysis of protein sequences, and the revolution in mass spectrometry.

List of contents

Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide Isolation
Purification of Proteins and Peptides By SDS-PAGE
Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose
Mass Spectrometric Strategies for the Structural Characterization of Proteins

Report

Reviews of the First Edition:
"Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --Cell

Product details

• Authors: Paul T. Matsudaira
• Assisted by: Paul T. Matsudaira (Editor), Paul T. (Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology Matsudaira (Editor), Matsudaira Paul T. (Editor)
• Publisher: ELSEVIER SCIENCE BV
• Languages: English
• Product format: Paperback / Softback
• Released: 01.01.2008
• EAN: 9780124802827
• ISBN: 978-0-12-480282-7
• No. of pages: 184
• Subjects: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics; SCIENCE / Life Sciences / Biochemistry, SCIENCE / Biotechnology, SCIENCE / Life Sciences / Cell Biology, biochemistry, biotechnology, proteins, Cellular biology (cytology)