Read more
This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.
List of contents
Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System.- Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing.- Myelin: Methods for Purification and Proteome Analysis.- In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells.- Isolation and Culture of Oligodendrocytes.- A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation.- Oligodendrocyte-Neuron Myelinating Co-Culture.- Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier.- Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices.- Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord.- Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination.- Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination.- A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation.- Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis.- Conditional Mutagenesis in Oligodendrocyte Lineage Cells.- Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage.- The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination.- Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease.- A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes.- Transmission Electron Microscopy of Oligodendrocytes and Myelin.- Toxin-Based Models to Investigate Demyelination and Remyelination.- Magnetic Resonance techniques for Imaging White Matter.
Summary
This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.
