Serum/Plasma Proteomics - Methods and Protocols

Over the past decade, there has been an increase in powerful proteomics technologies that allow greater fundamental insights into the blood proteome. Further developments in informatic analyses, software developments, and computational tools are providing insights into large data sets, open-source data along with large-scale application of bioinformatics. Serum/Plasma Proteomics: Methods and Protocols, Second Edition is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, covering fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays,  proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, data management, statistical design, and bioinformatic challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Serum/Plasma Proteomics: Methods and Protocols, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesicles/exosomes, and related biofluids.

List of contents

Direct Assessment of Plasma/Serum Sample Quality for Proteomics Biomarker Investigation.-  A Protocol for the Preparation of Cryoprecipitate and Cryo-Depleted Plasma.- A Proteomic Protocol for the Preparation of Platelet Concentrates.- Bead-based and Multiplexed Immunoassays for Protein Profiling via Sequential Affinity Capture.- Affinity Proteomics for Fast, Sensitive, Quantitative Analysis of Proteins in Plasma.- Characterization of the Low-molecular Weight Human Plasma Peptidome.- In-depth, Reproducible Analysis of Human Plasma using IgY 14 and SuperMix Immunodepletion.- Low-Molecular-Weight Plasma Proteome Analysis Using Top-Down Mass Spectrometry.- Identification of Post-Translational Modifications from Serum/Plasma by Immunoaffinity Enrichment and LC-MS/MS Analysis without Depletion of Abundant Proteins.- Identification of Core-fucosylated Glycoproteome in Human Plasma.- Proteomic Analysis of Blood Extracellular Vesicles in Cardiovascular Disease by LC-MS/MS Analysis.- Targeted Approach For Proteomic Analysis of a Hidden Membrane Protein.- Red Blood Cells in Clinical Proteomics.- High-throughput Quantitative Lipidomics Analysis of Non-esterified Fatty Acids in Plasma by LC-MS.- Simultaneous Enrichment of Plasma Extracellular Vesicles and Glycoproteome for Studying Disease Biomarkers.- Lipidomics of Human Blood Plasma by High Resolution Shotgun Mass Spectrometry.- Proteomics Analysis of Circulating Serum Exosomes.- High-density Serum/Plasma Reverse Phase Protein Arrays.- Antibody Colocalization Microarray for Cross-reactivity free Multiplexed Protein Analysis.- Surface Profiling of Extracellular Vesicles From Plasma or Ascites Fluid using Dotscan Antibody.- Serum Profiling for Identification of Autoantibody Signatures in Diseases using Protein Microarrays.- Quantitative Comparisons of Large Numbers of Human Plasma Samples using TMT10plex Labelling.- Efficient Quantitative Comparisons of Plasma Proteomes using Label-free Analysis with MaxQuant.- Blood and Plasma Proteomics: Targeted Quantitation and Post-translational Redox Modifications.- SWATH Mass Spectrometry for Proteomics of Non-depleted Plasma.- Shotgun and Targeted Plasma Proteomics to Predict Prognosis of Non-Small Cell Lung Cancer.- High-throughput Parallel Proteomic Sample Preparation using 96-well Polyvinylidene Fluoride (PVDF) Membranes and C18 Purification Plates.- Targeted Quantification of the Glycated Peptides of Human Serum Albumin.- Absolute Quantification of Middle to High Abundant Plasma Proteins via Targeted Proteomics.- A Highly Automated Shotgun Proteomic Workflow - Clinical Scale and Robustness for Biomarker Discovery in Blood.- Mass Spectrometry-based Serum Proteomics for Biomarker Discovery and Validation.- Metabolomics Towards Biomarker Discovery.- Plasma Biomarker Identification and Quantification by Microparticle Proteomics.- Bronchoalveolar Lavage Quantitative Mass Spectrometry based Proteomics Analysis in Lung Diseases.- Protein Multiplexed Immunoassay Analysis with R.- Appendix I Standard operating procedures for plasma collection in clinical research.- Appendix II Standard operating procedures for serum collection in clinical research.- Appendix III Reference ranges for blood tests. 

Summary

Over the past decade, there has been an increase in powerful proteomics technologies that allow greater fundamental insights into the blood proteome. Further developments in informatic analyses, software developments, and computational tools are providing insights into large data sets, open-source data along with large-scale application of bioinformatics. Serum/Plasma Proteomics: Methods and Protocols, Second Edition is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, covering fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays,  proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, data management, statistical design, and bioinformatic challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Serum/Plasma Proteomics: Methods and Protocols, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesicles/exosomes, and related biofluids.

Product details

• Assisted by: David W. Greening (Editor), J Simpson (Editor), J Simpson (Editor), Richard J. Simpson (Editor), Davi W Greening (Editor), David W Greening (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 31.07.2019
• EAN: 9781493983803
• ISBN: 978-1-4939-8380-3
• No. of pages: 551
• Dimensions: 179 mm x 32 mm x 255 mm
• Weight: 1068 g
• Illustrations: XVI, 551 p. 94 illus., 70 illus. in color.
• Series: Methods in Molecular Biology; Methods in Molecular Biology
• Subjects: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics; B, Life Sciences, proteins, Biomedical and Life Sciences, Protein Science