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This volume details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Chapters guide readers through an overview of disease-specific ncRNAs, computational methods and workflows for ncRNA discovery, annotation based on high-throughput sequencing data, bioinformatics tools and databases for ncRNA analyses, network-based methods, and kinetic modelling of ncRNA-mediated gene regulation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Computational Biology of Non-Coding RNA: Methods and Protocols aims to provide a state-of-the-art collection of computational methodsand approaches that will be of value to researchers interested in ncRNA field.
List of contents
ncRNAs in Inflammatory and Infectious Diseases.- p73-Governed miRNA Networks: Translating Bioinformatics Approaches to Therapeutic Solutions for Cancer Metastasis.- Methods for Annotation and Validation of Circular RNAs from RNAseq Data.- Methods to Study Long Non-coding RNA Expression and Dynamics in Zebrafish using RNA Sequencing.- Workflow Development for the Functional Characterization of ncRNAs.- ncRNA Editing: Functional Characterization and Computational Resources.- Computational Prediction of Functional microRNA - mRNA Interactions.- Tools for Understanding miRNA-mRNA Interactions for Reproducible RNA Analysis.- Computational Resources for Prediction and Analysis of Functional miRNA and their Targetome.- Non-coding RNAs Databases: Current Status and Trends.- Controllability Methods for Identifying Associations between Critical Control ncRNAs and Human Diseases.- Network-based Methods and Other Approaches for Predicting lncRNA Functions and Disease Associations.- Integration ofmiRNA and mRNA Expression Data for Understanding Etiology of Gynecologic Cancers.- Quantitative Characteristic of ncRNA Regulation in Gene Regulatory Networks.- Kinetic Modeling of Competition and Depletion of Shared miRNAs by Competing Endogenous RNAs.- Modeling ncRNA-mediated Circuits in Cell Fate Decision.- Modeling Long ncRNA-mediated Regulation in the Mammalian Cell Cycle.
Summary
Chapters guide readers through an overview of disease-specific ncRNAs, computational methods and workflows for ncRNA discovery, annotation based on high-throughput sequencing data, bioinformatics tools and databases for ncRNA analyses, network-based methods, and kinetic modelling of ncRNA-mediated gene regulation.
