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This volume aims at providing an update on state of the art methodologies to study various aspects of selenoprotein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Selenoproteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.
List of contents
SECISearch3 and Seblastian: In Silico Tools to Predict SECIS Elements and Selenoproteins.- Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins.- SelGenAmic: An Algorithm for Selenoprotein Gene Assembly.- Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification and Sequencing.- Identification and Characterization of Proteins that Bind to Selenoprotein 3' UTRs.- Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis.- Studying Selenoprotein mRNA Translation using RNA-Seq and Ribosome Profiling.- Modification of Selenoprotein mRNAs by Cap Tri-methylation.- Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS).- Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC - ICP MS).-Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum.- Radioactive 75Se Labeling and Detection of Selenoproteins.- Non-radioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines.- Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips.- Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues.- Overexpression of Recombinant Selenoproteins in E. coli.- Preparation of Selenocysteine-containing Forms of Human SELENOK and SELENOS.- Selenocysteine Mediated Expressed Protein Ligation of SELENOM.- Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity.- Selective Evaluation of Thioredoxin Reductase Enzymatic Activities.- Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk.- Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis.
Summary
This volume aims at providing an update on state of the art methodologies to study various aspects of selenoprotein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Selenoproteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.
