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This book focuses on the development and applications of functional nucleic acid-based detection methods in the context of food safety. Offering a comprehensive overview of nucleic acids detection method in food safety for professionals and members of the public interested in this area, the book is divided into two parts. Part I addresses the basic principle of nucleic acid detection, while Part II presents novel applications of detection methods in genetically modified organisms, the identification of dead-alive microorganisms, microbial diversity, heavy metal detection, gene toxicity and non-coding RNA identification. As such, it provides readers a wealth of knowledge on the use of nucleic acids as targets and media in food safety. It offers a valuable resource for clinicians and basic scientists in the areas of food science and microbiology, and for all those who are interested in the concrete applications of molecular biological techniques.
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List of contents
1 Introduction: a brief guide to targets and strategies of functional nucleic acids detection in food safety.- 2 Adaptable methods to extract nucleic acid targets and evaluate quality.- 3 Basic rules in nucleic acids-mediated amplification and hybridization methods in food safety detection: a review.- 4 Reference materials: a golden criterion in nucleic acids identification.- 5 Reference gene: in-species universality versus between-species uniquity.- 6 PCR-based technologies for identifying unknown gene sequences.- 7 A developed accurate digital PCR detection technology in food safety.- 8 Development of accurate nucleic acids detection technology for target quantification.- 9 Recent progress in high-throughput detection technology for food safety.- 10 Detecting targets without thermal cycling in food: isothermal amplification and hybridization.- 11 Aptamers and their application.- 12 Lateral Flow Nucleic Acid Biosensors.- 13 Nucleic Acid Biosensors for food safety.- 14 Alternative PCR methods for detecting GM crops & food in agriculture and the food chain: a review.- 15 The identification and detection technology of research in microorganism include living or dead bacteria.- 16 Characterization of microbial diversity in food chain: a molecular review.- 17 Identification and assessment of heavy metal pollution using nucleic acid-mediated technologies.- 18 Genotoxicity detection at the molecular level in food safety assessment: conventional methods and developments.- 19 A molecular review of the detection of specific nucleic acids by amplification and hybridization Characterization of microbial diversity in the food chain: a molecular review.
About the author
Dr. Wentao Xu is a PI (Principal Investigator) of Basic Platform for Food Safety in Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, China; an Associate Director in The Lab of Storage and Preservation ¿ Risk Assessment for Agri-products Quality and Safety (Beijing), MOA, China.
Summary
This book focuses on the development and applications of functional nucleic acid-based detection methods in the context of food safety. Offering a comprehensive overview of nucleic acids detection method in food safety for professionals and members of the public interested in this area, the book is divided into two parts. Part I addresses the basic principle of nucleic acid detection, while Part II presents novel applications of detection methods in genetically modified organisms, the identification of dead-alive microorganisms, microbial diversity, heavy metal detection, gene toxicity and non-coding RNA identification. As such, it provides readers a wealth of knowledge on the use of nucleic acids as targets and media in food safety. It offers a valuable resource for clinicians and basic scientists in the areas of food science and microbiology, and for all those who are interested in the concrete applications of molecular biological techniques.
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