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This volume provides protocols that revolve around three pillars of progress in the plant genomics field: genotypes, phenotypes, and the molecular processes in between. Chapters in Plant Genomics: Methods and Protocols are not restricted to the predominant model species Arabidopsis thaliana, hoping to encourage and facilitate other researchers to expand their research to other species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Genomics: Methods and Protocols aims to serve as an inspiration for further studies in plant genomics.
List of contents
CRISPR/Cas-mediated in planta Gene Targeting.- User Guide for the LORE1 Insertion Mutant Resource.- Enabling Reverse Genetics in Medicago truncatula Using High Throughput Sequencing for Tnt1 Flanking Sequence Recovery.- Generation of Doubled Haploid Lines for QTL Mapping.- Assessing Distribution and Variation of Genome-wide DNA Methylation using Short Read Sequencing.- Circular Chromosome Conformation Capture in Plants.- Genome Wide Profiling of Histone Modifications and Histone Variants in Arabidopsis thaliana and Marchantia polymorpha.- Tissue-Specific Transcriptome Profiling in Arabidopsis Roots.- Sample Preparation Protocols for Protein Abundance, Acetylome and Phosphoproteome Profiling of Plant Tissues.- Automated High-throughput Root Phenotyping of Arabidopsis thaliana under Nutrient Deficiency Conditions.- Large-scale Phenotyping of Root Traits in the Model Legume Lotus japonicas.- Long-term Confocal Imaging of Arabidopsis thaliana Roots for Simultaneous Quantification of Root Growth and Fluorescent Signals.- Identification of Protein-DNA Interactions Using Enhanced Yeast One-Hybrid Assays and a Semi-Automated Approach.- Mapping Protein- protein Interaction using High-throughput Yeast-2-Hybrid.- Mapping Protein-protein Interactions using Affinity Purification and Mass Spectrometry.- Measuring Protein Movement, Oligomerization State, and Protein-Protein Interaction in Arabidopsis Roots Using Scanning Fluorescence Correlation Spectroscopy (scanning FCS).- Studying Protein-Protein Interactions in planta using Advanced Fluorescence Microscopy.- Chemiluminescence Based Detection of Peptide Activity and Peptide-Receptor Binding in Plants.- Application of Chemical Genomics to Plant-Bacteria Communication: A High-Throughput System to Identify Novel Molecules Modulating the Induction of Bacterial Virulence Genes by Plant Signals.
Summary
This volume provides protocols that revolve around three pillars of progress in the plant genomics field: genotypes, phenotypes, and the molecular processes in between. Chapters in Plant Genomics: Methods and Protocols are not restricted to the predominant model species Arabidopsis thaliana, hoping to encourage and facilitate other researchers to expand their research to other species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Genomics: Methods and Protocols aims to serve as an inspiration for further studies in plant genomics.
