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This volume highlights the molecular and cellular methods used in studying Chronic Myeloid Leukimia (CML) pathogenesis and stem cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Chronic Myeloid Leukemia: Methods and Protocols aims to ensure successful results in the further study of this vital field.
List of contents
Molecular Detection of BCR-ABL in Chronic Myeloid Leukemia.- Induction of Chronic Myeloid Leukemia in Mice.- CML Mouse Model Generated from Leukemia Stem Cells.- Immunological Analyses of Leukemia Stem Cells.- Cell Cycle Analysis of CML Stem Cells using Hoechst33342 and Propidium Iodide.- Histological and In Vivo Microscopic Analysis of the Bone Marrow Microenvironment in a Murine Model of Chronic Myelogenous Leukemia.- The Culture Repopulation Ability (CRA) Assay and Incubation in Low Oxygen to Test Anti-Leukemic Drugs on Imatinib-resistant CML Stem-like Cells.- Tumor Suppressor Analysis in CML.- Detecting Autophagy and Autophagy Flux in Chronic Myeloid Leukemia Cells Using a Cyto ID Fluorescence Spectrophotometric Assay.- DREAM, A Simple Method for DNA Methylation Profiling by High Throughput Sequencing.- ChIP-seq Analysis of Human Chronic Myeloid Leukemia Cells.- Quantitative Proteomics Analysis of Leukemia Cells.- A Convenient Cell Culture Model for CML Acquired Resistance through BCR-ABL Mutations.- High Throughput Screening of Tyrosine Kinase Inhibitor Resistant Genes in CML.- Biological Analysis of Human CML Stem Cells; Xenograft Model of Chronic Phase Human Chronic Myeloid Leukemia.- New Mouse Models to Investigate the Efficacy of Drug Combinations in Human Chronic Myeloid Leukemia.- miRNome Analysis of CML Cells.- An Integrative Analysis of microRNA and mRNA Profiling in CML Stem Cells.- Single Cell Cytokine Profiling to Investigate Cellular Functional Diversity in Hematopoietic Malignancies.
Summary
This volume highlights the molecular and cellular methods used in studying Chronic Myeloid Leukimia (CML) pathogenesis and stem cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Chronic Myeloid Leukemia: Methods and Protocols aims to ensure successful results in the further study of this vital field.
