Rapid Cell Magnetisation Using Cationised Magnetoferritin

Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently. 

List of contents

Introduction.- Materials and Methods.- Characterising Magnetoferritin and Cationised Magnetoferritin.- Stem Cell Labelling with Cationised Magnetoferritin.- Toxicological Profiling of Cationised Magnetoferritin.- Escherichia coli labelling with Cationised Magnetoferritin.- Overall Conclusions and Future Opportunities.

Summary

Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently. 

Product details

• Authors: Sara Correia Carreira
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 01.01.2018
• EAN: 9783319868387
• ISBN: 978-3-31-986838-7
• No. of pages: 166
• Dimensions: 155 mm x 10 mm x 235 mm
• Weight: 289 g
• Illustrations: XVII, 166 p. 90 illus., 16 illus. in color.
• Series: Springer Theses; Springer Theses
• Subjects: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics; Nanotechnologie, B, Elektrizität, Magnetismus und Elektromagnetismus, Nanowissenschaften, Nanotechnology, Physics and Astronomy, Nanochemistry, Electricity, electromagnetism & magnetism, Magnetism, Magnetic materials, Magnetism, Magnetic Materials, Medical physics, Biophysics, Biological physics, Biological and Medical Physics, Biophysics, Genetics (non-medical), Stem Cell Biology, stem cells