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This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions. With a concentration on DNA methylation, the emphasis is on the most commonly studied epigenetic phenomenon that is most amenable to further study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Population Epigenetics: Methods and Protocols aims to aid population researchers in exploiting the latest insights into epigenetics to improve our understanding of the factors that influence human health and longevity.
List of contents
Library Construction for High-Throughput Mobile Element Identification and Genotyping.- The Design and Optimization of DNA Methylation Pyrosequencing Assays Targeting Region-Specific Repeat Elements.- Determining Epigenetic Targets: A Beginner's Guide to Identifying Genome Functionality Through Database Analysis.- Detecting Spatial Chromatin Organization by Chromosome Conformation Capture II: Genome-Wide Profiling by Hi-C.- Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method.- 5-Hydroxymethylcytosine Profiling in Human DNA.- Adjusting for Cell Type Composition in DNA Methylation Data Using a Regression-Based Approach.- Correcting for Sample Heterogeneity in Methylome-Wide Association Studies.- Nano-MeDIP-seq Methylome Analysis Using Low DNA Concentrations.- Bisulfite Conversion of DNA from Tissues, Cell Lines, Buffy Coat, FFPE Tissues, Microdissected Cells, Swabs, Sputum, Aspirates, Lavages, Effusions, Plasma, Serum, and Urine.- Analysis of Imprinted Gene Regulation.- Statistical Methods for Methylation Data.
Summary
This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions. With a concentration on DNA methylation, the emphasis is on the most commonly studied epigenetic phenomenon that is most amenable to further study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
