Skeletal Muscle Regeneration in the Mouse - Methods and Protocols

This volume focuseson the cell biology and physiology of skeletal muscle regeneration. This Bookis a collection of classic and cutting edge protocols optimized for mice, butin most cases adaptable to rat or other mammalian models, that will allow aninvestigator to develop and implement a research study on skeletal muscleregeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing theprogenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury byeccentric contraction; volumetric muscle loss; single myofiber isolation andculture; satellite cell transplantation; muscle clearing for whole mountimmunostaining; luciferase tracking of muscle stem cells; mitochondrialand mitophagy flux analysis; in vivo assessment of muscle contractility;force measurements on single isolated myofibers; and analysis of aerobicrespiration in intact skeletal muscle tissue by microplate respirometry. Written in the highlysuccessful Methods in Molecular Biology series format,chapters include introductions to each respective topic, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge andpractical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research inskeletal muscle growth, damage, repair, degeneration, and regenerative therapyin the mouse model system.

List of contents

EccentricContraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Modelsto Impair Skeletal Muscle's Capacity to Generate Force.- VolumetricMuscle Loss.- Freeze Injury of the Tibialis AnteriorMuscle.- Synergist Ablationas a Rodent Model to Study Satellite Cell Dynamics in Adult Skeletal Muscle.- Inducingand Evaluating Skeletal Muscle Injury by Notexin and Barium Chloride.- Cardiotoxin Induced Injury andSkeletal Muscle Regeneration.- Fibrosis-Inducing Strategies in Regenerating Dystrophic and Normal SkeletalMuscle.- Isolation, Cryosection, and Immunostainingof Skeletal Muscle.- Isolation of Mouse Periocular Tissuefor Histological and Immunostaining Analyses of the Extraocular Muscles andtheir Satellite Cells.- Skeletal Muscle Tissue Clearingfor LacZ and Fluorescent Reporters, and Immunofluorescence Staining.- Isolation, Culture, FunctionalAssays and Immunofluorescence of Myofiber-Associated Satellite Cells.- Transplantation-Based QuantitativeAssays for Satellite Cell Self-Renewal and Differentiation.- Non-Invasive Tracking of Quiescentand Activated MuSC Engraftment Dynamics In Vivo.- Myogenic Progenitors from Mouse PluripotentStem Cells for Muscle Regeneration.- Assaying Human Myogenic ProgenitorCell Activity by Reconstitution of Muscle Fibers and Satellite Cells in ImmunodeficientMice.- Methods for Mitochondria and MitophagyFlux Analyses in Stem Cells of Resting and Regenerating Skeletal Muscle.- Identification, Isolation, andCharacterization of Mesenchymal Progenitors in Mouse and Human Skeletal Muscle.- FACS Fractionation and Differentiation of Skeletal-MuscleResident Multipotent Tie2+ Progenitors.- In Vitro Assays to DetermineSkeletal Muscle Physiologic Function.- In Vivo Assessment of MuscleContractility in Animal Studies.- Functional Measurement ofRespiratory Muscle Motor Behaviors Using Transdiaphragmatic Pressure.- Assessment of the Contractile Propertiesof Permeabilized Skeletal Muscle Fibers.- Analysis of Aerobic Respirationin Intact Skeletal Muscle Tissue by Microplate-Based Respirometry.

About the author

Michael Kyba, Ph.D.
Associate Professor and Lillehei Endowed Scholar, Lillehei Heart Institute NHH4.126, University of Minnesota, SE
Minneapolis, MN 55455, USA

Summary

This volume focuses
on the cell biology and physiology of skeletal muscle regeneration. This Book
is a collection of classic and cutting edge protocols optimized for mice, but
in most cases adaptable to rat or other mammalian models, that will allow an
investigator to develop and implement a research study on skeletal muscle
regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the
progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by
eccentric contraction; volumetric muscle loss; single myofiber isolation and
culture; satellite cell transplantation; muscle clearing for whole mount
immunostaining; luciferase tracking of muscle stem cells; mitochondrial
and mitophagy flux analysis; in vivo assessment of muscle contractility;
force measurements on single isolated myofibers; and analysis of aerobic
respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly
successful Methods in Molecular Biology series format,
chapters include introductions to each respective topic, lists of the necessary
materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and
practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in
skeletal muscle growth, damage, repair, degeneration, and regenerative therapy
in the mouse model system.

Product details

• Assisted by: Michae Kyba (Editor), Michael Kyba (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 01.01.2018
• EAN: 9781493981458
• ISBN: 978-1-4939-8145-8
• No. of pages: 346
• Dimensions: 178 mm x 19 mm x 254 mm
• Weight: 677 g
• Illustrations: XII, 346 p. 97 illus., 87 illus. in color.
• Series: Methods in Molecular Biology; Methods in Molecular Biology
• Subjects: Natural sciences, medicine, IT, technology > Medicine > Clinical medicine; B, molecular biology, Biomedical and Life Sciences, Biomedical Research, Molecular Medicine