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This state-of-the-art laboratory manual includes 20 clinical protocols used daily for the investigation of the infertile male, presented with easy to understand, step-by-step methodology. The protocols are arranged from routine to advanced laboratory procedures common to clinical practice, including computer-assisted semen analysis, sperm preparation for IUI by density gradient and swim-up, sperm cryopreservation, and sperm DNA fragmentation test by TUNEL method, among others. The methodology in each protocol follows best practice guidelines made clearer by professionally hand-drawn illustrations covering most of the important steps and equipment. The authors, hailing from the world-renowned Andrology Center at Cleveland Clinic, have over 50 years of combined first-hand experience in managing very busy diagnostic and research facilities in male infertility and andrology. The book will be an indispensable resource for thousands of laboratory technologists, clinicians and reproductive professionals (andrologists, embryologist, etc.) engaged in the diagnosis and management of infertile men around the world.
List of contents
Diagnostic Tests inthe Evaluation of Male Infertility.- Quality Management inAndrology Laboratory.- Competency Assessmentin Andrology Laboratory.- Basic Semen Analysis.- Semen Analysis Using Hamilton-Thorn Casa.- Semen Analysis Using SCA (v5) For Casa.- Leukocytospermia Quantitation (ENDTZ) Test.- Eosin-Nigrosin Staining Procedure.- Sperm MorphologyStain (Diff-Quik®).- Qualitative SeminalFructose.- Cytospin Procedure $Nuclear Fast Red and Picroindigocarmine Stain Procedures For AzoospermiaSamples.- Hypo-Osmotic SwellingTest (HOS).- Procedure forRetrograde Ejaculate.- Sperm Preparation forIntrauterine Insemination Using Pureception®.- Sperm Preparation forIntrauterine Inseminvation (IUI) by Swim-Up Method.- Cryopreservation ofClient Depositor Semen.- Cryopreservation ofClient Depositor Semen Aspirate.- Cryopreservation ofClient Depositor Testicular Tissue.- Acrosome ReactionMeasurement.- Direct SpermmarAntibody Test.- Reactive OxygenSpecies (ROS) Measurement.- AntioxidantMeasurement in Seminal Plasma by TAC Assay.- DNA Damage inSpermatozoa (TUNEL) BD Accuri(TM) C6 Flow Cytometer.- Licensing andAccreditation of the Andrology Laboratory.
About the author
Ashok Agarwal, PhD, HCLD (ABB), ELD (ACE), Andrology Center and Reproductive Tissue Bank, American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
Sajal Gupta, MD (Ob/Gyn), MSc (Embryology), TS (ABB), Andrology Center and Reproductive Tissue Bank, American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
Rakesh Sharma, PhD, Andrology Center and Reproductive Tissue Bank, American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
Summary
This state-of-the-art laboratory manual includes 20 clinical protocols used daily for the investigation of the infertile male, presented with easy to understand, step-by-step methodology. The protocols are arranged from routine to advanced laboratory procedures common to clinical practice, including computer-assisted semen analysis, sperm preparation for IUI by density gradient and swim-up, sperm cryopreservation, and sperm DNA fragmentation test by TUNEL method, among others. The methodology in each protocol follows best practice guidelines made clearer by professionally hand-drawn illustrations covering most of the important steps and equipment. The authors, hailing from the world-renowned Andrology Center at Cleveland Clinic, have over 50 years of combined first-hand experience in managing very busy diagnostic and research facilities in male infertility and andrology. The book will be an indispensable resource for thousands of laboratory technologists, clinicians and reproductive professionals (andrologists, embryologist, etc.) engaged in the diagnosis and management of infertile men around the world.
