Read more
This book offers a comprehensive introduction to confocal microscopy - with a particular focus on spectral confocal microscopy. Beginning with an introduction to optical lenses, it provides a guide to compound microscopes and explains related topics like microscopic resolution. It then presents an outline of fluorescence and its corresponding implications for microscopy. The following excursus on the confocal beam paths includes implementation of acousto-optical devices and modern sensor techniques. Complex relationships are explained in a comprehensible manner, supported by many graphical figures.
Discussing the principles of magnifying optics and the technical fundamentals and modes of operation of modern laser scanning microscopes, it is a valuable resource for student and lab technicians as well as faculty members.
List of contents
Microscopy - Introduction.- Fluorescence.- Confocal Microscopy .- Light Sources.- Acousto Optical Excitation Filter.- White Beam Splitting.- Splitting of Emissions.- Emission Filtering.- Separation in the Time Domain.
About the author
Dr. Rolf Theodor Borlinghaus, born 1956, study of biology in Konstanz and promotion to Dr. rer. nat. at the biophysical institute. From 1990 product manager for complex fluorescence microscopy and confocal microscopy with Carl Zeiss Oberkochen (later Jena). Since 1997 with Leica Lasertechnik Heidelberg, now Leica Microsystems Mannheim. Today responsible for technology transfer and training.
Summary
This book offers a comprehensive introduction to confocal microscopy – with a particular focus on spectral confocal microscopy. Beginning with an introduction to optical lenses, it provides a guide to compound microscopes and explains related topics like microscopic resolution. It then presents an outline of fluorescence and its corresponding implications for microscopy. The following excursus on the confocal beam paths includes implementation of acousto-optical devices and modern sensor techniques. Complex relationships are explained in a comprehensible manner, supported by many graphical figures.
Discussing the principles of magnifying optics and the technical fundamentals and modes of operation of modern laser scanning microscopes, it is a valuable resource for student and lab technicians as well as faculty members.
