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This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications. Chapters guide the reader in the use of primary and immortalized cell lines, methodologies that exploit transcription and receptor mechanisms, newly developed animal models using nematodes, flies, amphioxus, frogs, zebrafish, and genetically engineered mice, and pre-clinical approaches to understanding BMP function in bone regeneration, heterotopic ossification, and cancers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bone Morphogenetic Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.
List of contents
Common Cell Lines Used to Study Bone Morphogenetic Proteins (BMPs).- In Vitro and In Vivo Osteogenic Differentiation of Human Adipose-Derived Stromal Cells.- Cell-based Gene Therapy System for Delivering BMPs.- Generation of Endogenous BMP Transcriptional Reporter Cells through CRISPR/Cas9 Genome Editing.- High-Throughput, Biosensor-based Approach to Examine Bone Morphogenetic Protein (BMP) - Receptor Interactions.- Mutagenesis and Imaging studies of BMP Signaling Mechanisms in C. elegans.- Gene Regulation of BMP Ligands in Drosophila.- Using Amphioxus as a Basal Chordate Model to Study BMP Signaling Pathway.- Proteolytic Activation of Bmps: Analysis of Cleavage in Xenopus Oocytes and Embryos.- Imaging and quantification of P-Smad1/5 in zebrafish blastula and gastrula embryos .- An Adult Zebrafish Model of Fibrodysplasia Ossificans Progressiva.- Generation and Identification of Genetically Modified Mice for BMP Receptors.- Phenotypic Analyses of Genetically Modified Mice for BMP Receptors.- Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-embedded Tissue Sections.- Spatial and Quantitative Detection of BMP Activity in Mouse Embryonic Limb Buds.- Pharmacologic Strategies for Assaying BMP Signaling Function.- Bone Morphogenetic Proteins (BMPs) and Bone Regeneration.- Heterotopic Ossification in Mouse Models of Fibrodysplasia Ossificans Progressiva.- Double-Humanized Mouse Model to Study Bone Morphogenetic Protein (BMP) Signaling in Tumor Xenografts.
Summary
This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications. Chapters guide the reader in the use of primary and immortalized cell lines, methodologies that exploit transcription and receptor mechanisms, newly developed animal models using nematodes, flies, amphioxus, frogs, zebrafish, and genetically engineered mice, and pre-clinical approaches to understanding BMP function in bone regeneration, heterotopic ossification, and cancers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bone Morphogenetic Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.
