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This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis.
List of contents
Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS).- Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells.- Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD.- Chromatin Immunoprecipitation in Skeletal Myoblasts.- Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor.- Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation.- Lentivirus-Mediated RNAi in Skeletal Myogenesis.- Adipogenesis from Bovine Precursors.- Transcriptomic Profiling during Myogenesis.- Co-Culture Method to Obtain Endothelial Networks within Human Tissue-Engineered Skeletal Muscle.- Interaction between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System.- LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue.- siRNA Gene Silencing during C2C12 Myogenesis.- Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury.- Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis.- Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis.- Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers.- Myoblast Phosphoproteomics as a Tool to Investigate Global Signalling Events during Myogenesis.- Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies.- RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis.
Summary
This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis.
