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This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Antibiotics: Methods and Protocols guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Antibiotics: Methods and Protocols aims to inspire scientific work in the exciting field of antibiotic research.
List of contents
Antibiotics - Precious Goods in Changing Times.- Mining Bacterial Genomes for Secondary Metabolite Gene Clusters.- Production of Antimicrobial Compounds by Fermentation.- Structure Elucidation of Antibiotics by NMR Spectroscopy.- Computer-Aided Drug Design Methods.- Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents.- Application of a Bacillus subtilis Whole-cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds.- Determination of Bacterial Membrane Impairment by Antimicrobial Agents.- Mass-sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes.- Measurement of Cell Membrane Fluidity by Laurdan GP:Fluorescence Spectroscopy and Microscopy.- In vitro Assays to Identify Antibiotics Targeting DNA Metabolism.- Fluorescence-based real-time Activity Assays to Identify RNase P inhibitors.- Reporter Gene-based Screening for TPP Riboswitch Activators.- Cell-based Fluorescent Screen to Identify Inhibitors of Bacterial Translation Initiation.-Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor Screen.- Expression Profiling of Antibiotic Resistant Bacteria Obtained by Laboratory Evolution.- Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research.- Label-Free Quantitation of Ribosomal Proteins from Bacillus Subtillis for Antibiotic Research.- Functional Metagenomics to Study Antibiotic Resistance.- Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains using High Throughput Sequencing.
Summary
This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Antibiotics: Methods and Protocols guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Antibiotics: Methods and Protocols aims to inspire scientific work in the exciting field of antibiotic research.
