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Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity. This goes against the long-held tenet that stem cells, defined by their capacity for self-renewal and lineage development, comprised a homogenous population, thus providing the reader with a new avenue of exploration into the complex world of stem cell study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Heterogeneity: Methods and Protocols serves as an ideal guide for investigators exploring this important area of research.
List of contents
The Heterogeneity of Stem Cells: A Brief Overview.- Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells.- An Effective and Reliable Xeno-Free Cryopreservation Protocol for Single Human Pluripotent Stem Cells.- Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter.- Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells.- Dissecting Transcriptional Heterogeneity in Pluripotency: Single Cell Analysis of Mouse Embryonic Stem Cells.- Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs).- Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice.- Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing.- Stencil Micropatterning for Spatial Control of Human Pluripotent Stem Cell Fate Heterogeneity.- Visualizing the Functional Heterogeneity of Muscle Stem Cells.- Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies.- Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells.- Growth Factor-Free Pre-Vascularization of Cell Sheets for Tissue Engineering.- In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry.- Aerosol-Based Cell Therapy for Treatment of Lung Diseases.- Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro.- Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro.- Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro.- Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method.- Automated Cell-Based Quantitation of 8-OHdG Damage.- CoCl2 Administration to Vascular MSC Cultures as an In Vitro Hypoxic System to Study Stem Cell Survival and Angiogenesis.- Reporter Systems to Study Cancer Stem Cells.- Agent-Based Modeling of Cancer Stem Cell Driven Solid Tumor Growth.- Induction of a Tumor-Metastasis-Receptive Microenvironment as an Unwanted Side Effect After Radio/Chemotherapy and In Vitro and In Vivo Assays to Study This Phenomenon.- Isolation and Propagation of Glioma Stem Cells from Acutely Resected Tumors.- Isolation and Characterization of Cancer Stem Cells of the Non-Small Cell Lung Cancer (A549) Cell Line.
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Summary
Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity. This goes against the long-held tenet that stem cells, defined by their capacity for self-renewal and lineage development, comprised a homogenous population, thus providing the reader with a new avenue of exploration into the complex world of stem cell study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Heterogeneity: Methods and Protocols serves as an ideal guide for investigators exploring this important area of research.
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