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Thisvolume provides a variety of protocols to analyze various epigeneticchanges, including differential expression of non-coding RNAs, changes in DNAmethylation, and histone modifications in plants. Chapters detail protocolswith different degrees of complexity, and describe bioinformatics approachesfor data processing and analysis. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritative andcutting-edge, Plant Epigenetics: Methods and Protocols, Second Editionaims to ensure successful results in the further study of this vital field.
List of contents
Chromatin ImmunoprecipitationProtocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis.- Chromatin Conformation Capture-Based Analysis of Nuclear Architecture.- Metaanalysis of Genome-Wide Chromatin Data.- Localization of Mirnas by In Situ Hybridization in Plants using ConventionalOligonucleotide Probes.- COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis ofLocus-Specific Changes in Methylation Patterns.- Analysis of Global Genome Methylation Using the Cytosine-Extension Assay.- In Situ Analysis Of DNA Methylation In Plants.- Analysis of DNA Hydroxymethylation In Arabidopsis UsingColorimetric Assay.- Analysis of DNA Cytosine Methylation Patterns usingMethylation Specific Amplification Polymorphism (MSAP).- Differentially MethylatedRegion - Representational Difference Analysis (DMR-RDA): A Powerful Method to IdentifyDmrs in Uncharacterized Genomes.- Analysis of Small RNA PopulationsUsingHybridization to DNA Tiling Arrays.- Northern Blotting Techniques for Small RNAs.- Stem-Loop Qrt-PCR for the Detection of PlantMicrornas.- Profiling New Small RNA Sequences.- Small RNA Library Preparation and Illumina Sequencing in Plants.- Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow.- Increasinga Stable Transformation Efficiency of Arabidopsis by Manipulating theEndogenous Gene Expression using Virus-Induced Gene Silencing.- The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNAStrand Breaks.- Profiling Transposable Elements and their Epigenetic Effects in Non-ModelSpecies.
Summary
This
volume provides a variety of protocols to analyze various epigenetic
changes, including differential expression of non-coding RNAs, changes in DNA
methylation, and histone modifications in plants. Chapters detail protocols
with different degrees of complexity, and describe bioinformatics approaches
for data processing and analysis. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Authoritative and
cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition
aims to ensure successful results in the further study of this vital field.
