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This book offers in a single volume a unique collection of the state-of the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and a variety of experimental models including cultured mammalian cells, laboratory mice, and Drosophila melanogaster, as well as offering a description of high throughput approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Oncogene-Induced Senescence: Methods and Protocols represents a valuable asset for a wide audience of medical oncologists and researchers in the fields of oncology, molecular and cellular biology, biochemistry, and animal development.
List of contents
The Immortal Senescence.- Senescence Phenotypes Induced by Ras in Primary Cells.- Cellular Model of p21-Induced Senescence.- Detecting Markers of Therapy-Induced Senescence in Cancer Cells.- Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence.- Detection of Dysfunctional Telomeres in Oncogene-Induced Senescence.- RT-qPCR Detection of Senescence-Associated Circular RNAs.- Autophagy Detection During Oncogene-Induced Senescence Using Fluorescence Microscopy.- Detecting the Senescence-Associated Secretory Phenotype (SASP) by High Content Microscopy Analysis.- Sudan Black B, the Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells.- Using [U-13C6]-Glucose Tracer to Study Metabolic Changes in Oncogene-Induced Senescence Fibroblasts.- Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence.- Detection of Reactive Oxygen Species inCells Undergoing Oncogene-Induced Senescence.- Detection of Senescent Cells by Extracellular Markers Using a Flow Cytometry-Based Approach.- Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senescence.- Detection of Nucleotide Disbalance in Cells Undergoing Oncogene-Induced Senescence.- Senescence-Like Phenotypes in Human Nevi.- Detection of Oncogene-Induced Senescence In Vivo.- Detection of Senescence Markers During Mammalian Embryonic Development.- Induction and Detection of Oncogene-Induced Cellular Senescence in Drosophila.
Summary
This book offers in a single volume a unique collection of the state-of the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and a variety of experimental models including cultured mammalian cells, laboratory mice, and Drosophila melanogaster, as well as offering a description of high throughput approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Oncogene-Induced Senescence: Methods and Protocols represents a valuable asset for a wide audience of medical oncologists and researchers in the fields of oncology, molecular and cellular biology, biochemistry, and animal development.
