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This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.
List of contents
Generating Genetically Modified Mice: A Decision Guide.- Direct Generation of Conditional Alleles using CRISPR/Cas9 in Mouse Zygotes.- Building Cre Knockin Rat Lines using CRISPR/Cas9.- Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases.- Use of the DICE Dual Integrase Cassette Exchange System.- Ligand-controlled Site-specific Recombination in Zebrafish.- Injection-based Delivery of Cell Permeable Peptide-tagged Cre.- Viral Delivery of GFP-dependent Recombinases to the Mouse Brain.- Recombinase-mediated Cassette Exchange using Adenoviral Vectors.- Marker Removal in Transgenic Plants using Cre recombinase Delivered with Potato Virus X.- Nanoparticle-mediated Recombinase Delivery into Maize.- Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines.- FLPing Genes On and Off in Drosophila.- Imaging Neural Architecture in Brainbow Samples.- Purification and in vitro characterization.
Summary
This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.
