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Thisvolume aims to describe acomplementary range of molecular, cell biological, and in vivo protocols used to investigatethe structure-function of nuclear receptors, together with experimentalapproaches that may lead to new drugs to selectively target nuclearreceptor-associated diseases. The NuclearReceptor Superfamily, Second Edition will benefit those starting out in thenuclear receptor research field as well as to more established researchers whowish to apply different methods to a particular receptor or research problem.Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritativeand cutting-edge, The Nuclear ReceptorSuperfamily, Second Edition aims to ensure successful results in thefurther study of this vital field.
List of contents
The Nuclear Receptor Superfamily- AtThirty.- Lipid Homeostasis and Ligands forLiver X Receptors: Identification and Characterization.- Useof Differential Scanning Fluorimetry to Identify Nuclear Receptor Ligands.- Drug-Discovery Pipeline for NovelInhibitors of the Androgen Receptor.- Useof BRET to Study Protein-Protein Interactions in vitro and in vivo.- StudyingSteroid Receptor Complexes in the Cellular Environment.- Post-translationalModifications of Steroid Receptors: Phosphorylation.- Mapping protein-DNA Interactions using ChIP-exo and Illumina-based Sequencing.- Methods to IdentifyChromatin Bound Protein Complexes: From Genome-Wide to Locus Specific Approaches.- Measuring the Expression of microRNAs Regulated byAndrogens.- Methods for Identifying andQuantifying Mrna Expression of Androgen Receptor Splicing Variants in ProstateCancer.- HarvestingHuman Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells.- Invivo Imaging of Nuclear Receptor Transcriptional Activity.- Developmentand Characterisation of Cell-Specific Androgen Receptor Knockout Mice.
Summary
The NuclearReceptor Superfamily, Second Edition will benefit those starting out in thenuclear receptor research field as well as to more established researchers whowish to apply different methods to a particular receptor or research problem.
