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This volume is a collection ofresearch methods, techniques, and approaches to investigate the molecularbiology of West Nile Virus (WNV). Chapters in the book cover many facets ofWNV, such as propagation and titration of WNV in vero cells, examination of WNVneuroinvasion and neuropathogenesis in the central nervous system of a murinemodel, field surveillance methods for WNV, and in vitro and in vivoblood-brain barrier models to study WNV pathogenesis. A brief introduction,along with a discussion on WNV laboratory safety are also included. Written inthe highly successful Methods in Molecular Biology series format,chapters includes introduction to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, West Nile Virus will interest virologists,molecular biologists, microbiologists, and other scientists working with WNV.This book is a comprehensive guide for those conducting research in the fieldof WNV biology, including life cycles, pathogenesis, impact of infection, andpossible treatment/prevention development.
List of contents
A Brief Reviewof West Nile Virus Biology. Overviewof West Nile Virus Transmission and Epidemiology.- Propagationand Titration of West Nile Virus on Vero Cells.- Generating West Nile Virus from anInfectious Clone.- FunctionalAnalysis of West Nile Virus Proteins in Human Cells.- Lessonsfrom the Murine Models of West Nile Virus Infection.- Explorationof West Nile Virus Infection in Mouse Models.- Examinationof West Nile Virus Neuroinvasion and Neuropathogenesis in the Central Nervous Systemof a Murine Model.- In Vitro and In Vivo Blood-Brain Barrier Models to Study West Nile Virus Pathogenesis.- Methodology for Identifying Host Factors Involvedin West Nile Virus Infection.- ELISA andNeutralization Methods to Measure Anti-West Nile Virus Antibody Responses.- Safe Handlingof West Nile Virus in the Insectary.- Techniques for Experimental Infection of Mosquitoeswith West Nile Virus.- Field Surveillance Methods for West Nile Virus.- DetectionProtocols for West Nile Virus in Mosquitoes, Birds, and Non-Human Mammals.- Field Methods and Sample Collection Techniquesfor the Surveillance of West Nile Virus in Avian Hosts.- StatisticalTools for the Interpretation of Enzootic West Nile Virus Transmission Dynamics.- Role of ImmuneAging in Susceptibility to West Nile Virus.- An Overviewof Current Approaches Toward the Treatment and Prevention of West Nile Virus Infection.
Summary
This volume is a collection of
research methods, techniques, and approaches to investigate the molecular
biology of West Nile Virus (WNV). Chapters in the book cover many facets of
WNV, such as propagation and titration of WNV in vero cells, examination of WNV
neuroinvasion and neuropathogenesis in the central nervous system of a murine
model, field surveillance methods for WNV, and in vitro and in vivo
blood-brain barrier models to study WNV pathogenesis. A brief introduction,
along with a discussion on WNV laboratory safety are also included. Written in
the highly successful Methods in Molecular Biology series format,
chapters includes introduction to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, West Nile Virus will interest virologists,
molecular biologists, microbiologists, and other scientists working with WNV.
This book is a comprehensive guide for those conducting research in the field
of WNV biology, including life cycles, pathogenesis, impact of infection, and
possible treatment/prevention development.
