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Inaddition to updating important core techniques this third edition presents newchapters on assessing leukocyte involvement in angiogenesis, lymphatic cell andpericyte isolation techniques, spheroid and arterial ring based in vitroassays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols,Third Edition also guides readers through new protocols on chorioallantoicmembrane models, corneal pocket assays to assess angio- and lymphangiogenesis,models of muscle angiogenesis, and use of zebrafish embryos to study vascularangiogenesis and senescence. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritativeand practical, Angiogenesis Protocols, Third Edition provides not only apractical handbook for key techniques, but also an informative and enjoyableread for all those interested, no matter how directly, in angiogenesis.
List of contents
Assessing Tumor Angiogenesis in Histological Samples.- ImmunohistochemicalMethods for Measuring Tissue Lymphangiogenesis.- Immunohistochemical Assessmentof Leukocyte Involvement in Angiogenesis.- Isolation and Culture of HumanEndothelial Cells from Micro- And Macro-Vessels.- Isolation, Identification andCulture of Human Lymphatic Endothelial Cells.- Isolation, Culture andCharacterisation Of Vascular Smooth Muscle Cells.- Isolation and Transfection ofPrimary Culture Bovine Retinal Pericytes.- In Vitro Assays for Endothelial CellFunctions Required for Angiogenesis: Proliferation, Motility, TubularDifferentiation And Matrix Proteolysis.- Tube Forming Assays.- In VitroCo-Culture Assays of Angiogenesis.- Spheroid-Based In Vitro Angiogenesis Model.-Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A NovelIn Vitro Model for the Study Of Angiogenesis.- Human Arterial Ring AngiogenesisAssay.- A Modified Aortic Ring Assay to Assess Angiogenic Potential InVitro.- QuantitativeImaging-Based Examination of Pericyte Control of Endothelial Growth Dynamics andAngiogenesis.- Static and Dynamic Assays of Cell Adhesion Relevant to theVasculature.- Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesisby Intravital Microscopy.- Quantitative Estimation of Tissue Blood Flow Rate.- ChorioallantoicMembrane Microtumor Model to Study the Mechanisms of Tumor Angiogenesis,Vascular Permeability, and Tumor Cell Intravasation.- The Rabbit Corneal PocketAssay.- The Corneal Micropocket Assay: AModel of Angiogenesis and Lymphangiogenesis.- Models of Oxygen InducedRetinopathy in Rodents.- The Sponge Implant Model of Angiogenesis.- Measurementof Angiogenesis, Arteriolargenesis and Lymphangiogenesis Phenotypes by Use ofTwo-Dimensional Mesenteric Angiogenesis Assay.- In Vivo Models of MuscleAngiogenes.- Use of the Hollow Fibre Assay to Evaluate Agents that Target TheTumor Neovasculature.- Angiogenesis and Senescence in Zebrafish Embryos.
Summary
Inaddition to updating important core techniques this third edition presents newchapters on assessing leukocyte involvement in angiogenesis, lymphatic cell andpericyte isolation techniques, spheroid and arterial ring based in vitroassays, and on pericyte involvement in angiogenesis.
