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Thisvolume covers multidisciplinary approaches on various aspects of ChikungunyaVirus (CHIKV) research that was brought together from leading laboratoriesacross the globe. Topics covered include techniques in clinical and diagnosticvirology, basic protocols in cell and virus culture, bioinformatics, andproteomics approaches in cellular response studies. Chikungunya Virus:Methods and Protocols also discusses methods in immunology and animal modelstudies, as well as different strategies of antivirals and vaccine developmentfor therapeutics against CHIKV infection. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary material and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.
Thorough and practical, Chikungunya Virus: Methods and Protocols is auseful and comprehensive resource for CHIKV researchers and anyone interestedin learning more about the Chikungunya Virus.
List of contents
Evolution and Epidemiologyof Chikungunya Virus.- Detectionand Quantification of Chikungunya Virus by Real-Time RT-PCR Assay.- Advanced Genetic Methodologiesin Tracking Evolution and Spread of Chikungunya Virus.- SyntheticPeptide-Based Antibody Detection for Diagnosis of Chikungunya Infection withand without Neurological Complications.- Expression and Purification of E2 Glycoprotein from InsectCells (Sf9) for Use in Serology.- Diagnostic Methods for CHIKV Based on Serological Tools.- Utilization and Assessment of Throat Swab and Urine Specimensfor Diagnosis of Chikungunya Virus Infection.- Propagationof Chikungunya Virus Using Mosquito Cells.- InfectiousViral Quantification of Chikungunya Virus - Virus Plaque Assay.- MolecularEpidemiology of Chikungunya Virus by Sequencing.- Chikungunya Virus Infection of Aedes Mosquitoes.- Analysisof CHIKV in Mosquitoes Infected via Artificial Blood Meal.- ChikungunyaVirus Growth and Fluorescent Labeling: Detection of Chikungunya Virus by ImmunofluorescenceAssay.- Virus Isolation and Preparation of Sucrose-BandedChikungunya Virus Samples for Transmission Electron Microscopy.- Viral-HostProtein Interaction Studies Using Yeast Two-Hybrid Screening Method.- Application of GelC-MS/MS to Proteomic Profiling of Chikungunya Virus Infection:Preparation of Peptides for Analysis.- BioinformaticsBased Approaches to Study Virus-Host Interactions During Chikungunya Virus Infection.- T-Cell Epitope Prediction ofChikungunya Virus.- MouseModels of Chikungunya Virus.- Generationof Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HYHybridoma Cloning Kit.- ImmunohistochemicalDetection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-EmbeddedTissues.- Antiviral Strategies against Chikungunya Virus.- A Real Time Cell Analyzing Assay for Identificationof Novel Antiviral Compounds against Chikungunya Virus.- Using Bi-Cistronic Baculovirus Expression Vector System to Screen the Compoundsthat Interfere the Infection of Chikungunya Virus.- NeutralizationAssay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test.- ReverseGenetics Approaches for Chikungunya Virus.- Productionof Chikungunya Virus-Like Particles and Subunit Vaccines in Insect Cells.- Protocols for Developing Novel Chikungunya Virus DNAVaccines.
Summary
This
volume covers multidisciplinary approaches on various aspects of Chikungunya
Virus (CHIKV) research that was brought together from leading laboratories
across the globe. Topics covered include techniques in clinical and diagnostic
virology, basic protocols in cell and virus culture, bioinformatics, and
proteomics approaches in cellular response studies. Chikungunya Virus:
Methods and Protocols also discusses methods in immunology and animal model
studies, as well as different strategies of antivirals and vaccine development
for therapeutics against CHIKV infection. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary material and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls.
Thorough and practical, Chikungunya Virus: Methods and Protocols is a
useful and comprehensive resource for CHIKV researchers and anyone interested
in learning more about the Chikungunya Virus.
