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This detailed book compiles state-of-the-art protocols from researchers actively working in the area of autophagy, a crucial cellular process that regulates numerous cellular functions. In order to accelerate advances in the field, the volume explores aspects of autophagy research where a better understanding of its role is vitally important, such as in the maintenance of stem cell subpopulations and the regulation of differentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Autophagy in Differentiation and Tissue Maintenance: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of this key cell biological action.
List of contents
Visualization and Measurement of Multiple Components of the Autophagy Flux.- Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells.- Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells.- Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement.- Autophagy in Adipocyte Differentiation.- Assessing Autophagy in the Leydig Cells.- Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells.- Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-Small Cell Lung Cancer Cells.- Autophagy in Zebrafish Extraocular Muscle Regeneration.- The Detection Techniques for Autophagy-Associated Cell Death Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry.- Cloning of Autophagy-Related MicroRNAs.- Assays to Monitor Aggrephagy in Drosophila Brain.- Methods for Monitoring Autophagy in Silkworm Organs.- Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia.- Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening.- Porcine Cell-Free System to Study Mammalian Sperm Mitophagy.- Monitoring and Measuring Mammalian Autophagy.
Summary
This detailed book compiles state-of-the-art protocols from researchers actively working in the area of autophagy, a crucial cellular process that regulates numerous cellular functions. In order to accelerate advances in the field, the volume explores aspects of autophagy research where a better understanding of its role is vitally important, such as in the maintenance of stem cell subpopulations and the regulation of differentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Autophagy in Differentiation and Tissue Maintenance: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of this key cell biological action.
