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This volume describes prominentmethodologies developed by laboratories that have been leading the field ofquantitative proteomics by mass spectrometry. The procedures for performing theexperiments are described in an easy-to-understand manner with many technicaldetails that usually are not reported in typical research articles. This secondedition of Quantitative Proteomics by Mass Spectrometry provides a broad perspective of the methodologiesused for quantifying proteins and post-translational modifications in differenttypes of biomedical specimens. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritative and thorough, QuantitativeProteomics by Mass Spectrometry, Second Edition is avaluable resource to help researchers understand and learn about thelatest tools used in the study of quantitative proteomics by mass spectrometry.
List of contents
IncreasedDepth and Breadth of Plasma Protein Quantitation via Two Dimensional LiquidChromatography/Multiple Reaction Monitoring-Mass Spectrometry with LabeledPeptide Standards.- QuantitativeAnalysis of the Wirt5-Regulated Lysine Succinylation Proteome in MammalianCells.- Determiningthe Composition and Stability of Protein Complexes Using an Integrated Label-Freeand Stable Isotope Labeling Strategy.- Label-FreeQuantitation for Clinical Proteomics.- ProteogenomicMethods to Improve Genome Annotation.- MassSpectrometry-Based Quantitative O-GlcNAcomic Analysis.- Isolating andQuantifying Plasma HDL Proteins by Sequential Density GradientUltracentrifugation and Targeted Proteomics.- A Method forLabel-Free Differential Top-Down Proteomics.- Multiplexed Immunoaffinity Enrichment ofPeptides with Anti-Peptide Antibodies and Quantification by Stable IsotopeDilution Multiple Reaction Monitoring Mass Spectrometry.- HighThroughput Quantitative Proteomics Enabled by Mass Defect-Based 12-Plex DiLeuIsobaric Tags.- IsotopicN, N-Dimethyl Leucine (iDiLeu) for Absolute Quantification of Peptides Using aStandard Curve Approach.- SelectingOptimal Peptides for Targeted Proteomic Experiments in Human Plasma Using In Vitro Synthesized Proteins asAnalytical Standards.- Usingthe CPTAC Assay Portal to Identify and Implement Highly Characterized TargetedProteomics Assays.- Large-Scaleand Deep Quantitative Proteome Profiling Using Isobaric Lableing Coupled withTwo-Dimensional LC-MS/MS.- Multipleand Selective Reaction Monitoring Using Triple Quadrupole Mass Spectrometer:Pre-Clinical Large Cohort Analysis.- Methodsfor SWATH(TM): Data Independent Acquisition on TripleTOF Mass Spectrometers.- Measurementof Phosphorylated Peptides with Absolute Quantification.- Proteomic Analysis of Protein Turnover by Metabolic Whole Rodent Pulse-Chase Isotopic Labeling and Shotgun Mass Spectrometry Analysis.
Summary
This volume describes prominent
methodologies developed by laboratories that have been leading the field of
quantitative proteomics by mass spectrometry. The procedures for performing the
experiments are described in an easy-to-understand manner with many technical
details that usually are not reported in typical research articles. This second
edition of Quantitative Proteomics by Mass Spectrometry provides a broad perspective of the methodologies
used for quantifying proteins and post-translational modifications in different
types of biomedical specimens. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Authoritative and thorough, Quantitative
Proteomics by Mass Spectrometry, Second Edition is a
valuable resource to help researchers understand and learn about the
latest tools used in the study of quantitative proteomics by mass spectrometry.
