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This volumeexplores the evolution of bacterial cancer therapy and describes the moderntechniques used in therapy today. The chapters in this book cover a broad rangeof topics such as the development of tumor-targeting Salmonella typhimurium A1-R, a microfluidic device for precise quantification of the interactions betweentumor-targeting bacteria and tumor tissue, non-invasive in vivo imaging ofbacteria-mediated cancer therapy using bio-luminescent bacteria, methods toachieve remote-control of therapeutic gene expression in tumor-targetingbacteria, and cell-cycle decoy of cancer cells resistant to cytotoxic drugs todrug sensitivity by S. typhimurium A1-R. This book concludes witha chapter on the future potential of bacterial therapy of cancer. Written inthe highly successful Methods in Molecular Biology series format,chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Thoroughand informative, Bacterial Therapy of Cancer: Methods and Protocols isa valuable resource for anyone who is interested in cancer and bacterialtherapy.
List of contents
Tumor Targeting Salmonella Typhimurium A1-R: An Overview.- Enhancement of Tumor-Targeted Delivery ofBacteria with Nitroglycerin Involving Augmentation of the ERP Effect.- Oral Delivery of Tumor-Targeting Salmonella to Treat Cancer in Mice.- Microfluidic Device to Quantify the Behavior ofTherapeutic Bacteria in Three-Dimensional Tumor Tissue.- Tumor-Targeting Therapy Using Gene-EngineeredAnaerobic-Nonpathogenic BifidobacteriumLongum.- Noninvasive InVivo Imaging to Follow Bacteria Engaged in Cancer Therapy.- In VivoBioluminescence Imaging of Intra-Tumoral Bacteria.- Employment of Salmonella in Cancer Gene Therapy.- Development of a Targeted Gene Delivery SystemUsing Escherichia Coli.- Isolation and Analysis of Suppressor Mutationsin Tumor-Targeted msbB Salmonella.- Determination of Plasmid Segregational Stabilityin a Growing Bacterial Population.- Visualization of Anti-Cancer Salmonella Typhimurium Engineered forRemote-Control of Therapeutic Proteins.- Methods for Tumor Targeting with Salmonella Typhimurium A1-R.- SalmonellaTyphimurium A1-R and Cell-Cycle Decoy Therapy of Cancer.- Future of Bacterial Therapy of Cancer.
About the author
Robert M. Hoffman, Ph.D.
Professor of Surgery, University of California, San Diego
President, AntiCancer, Inc.
7917 Ostrow Street
San Diego, CA 92111
USA
TEL: 1-858-654-2555
FAX: 1-858-268-4175
Email: all@anticancer.com
Summary
This volume
explores the evolution of bacterial cancer therapy and describes the modern
techniques used in therapy today. The chapters in this book cover a broad range
of topics such as the development of tumor-targeting Salmonella typhimurium A1-R, a microfluidic device for precise quantification of the interactions between
tumor-targeting bacteria and tumor tissue, non-invasive in vivo imaging of
bacteria-mediated cancer therapy using bio-luminescent bacteria, methods to
achieve remote-control of therapeutic gene expression in tumor-targeting
bacteria, and cell-cycle decoy of cancer cells resistant to cytotoxic drugs to
drug sensitivity by S. typhimurium A1-R. This book concludes with
a chapter on the future potential of bacterial therapy of cancer. Written in
the highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough
and informative, Bacterial Therapy of Cancer: Methods and Protocols is
a valuable resource for anyone who is interested in cancer and bacterial
therapy.
