Toll-Like Receptors - Practice and Methods

This second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction with other cellular and biologic systems, and cancer progression. This book is divided into five sections: Part I outlines methods for TLR detection, interaction, and intracellular trafficking; Part II describes methods and assays to investigate how TLRs cross-prime other pattern recognition receptors, including intracellular DNA receptors and inflammasome formation; Part III highlights RNA regulation, detailing how TLRs can induce RNA transcripts and molecules such as lncRNAs and microRNAs; Part IV explores TLR detection and activation in systems such as epithelial barrier function, metabolism and the circadian clock, as well as cellular systems including T and B lymphocytes; and Part V describes models to delineate the role of TLRs in diseases such as dermatitis, arthritis, and gastric cancer. Written in the highly successful Methods in Molecular Biology series format, each chapter contains a summary, a list of required materials, step-by-step, readily reproducible laboratory protocols, useful notes to investigate TLRs in cell culture, systems and disease, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Toll-Like Receptors: Methods and Protocols, Second Edition is a valuable resource to any immunologist, molecular or medical biologist working in a laboratory setting. It will add skill to both students and the more advanced molecular biologist who wishes to learn a new technique or move to a different area within their current repertoire of practical knowledge.

List of contents

Toll-LikeReceptors: Ligands, Cell-Based Models, and Readouts for ReceptorAction.- Bioinformatic Analysis of Toll-Like Receptor Sequences andStructures.- Toll-Like Receptor Interactions Measured by Microscopic andFlow Cytometric FRET.- Using Confocal Microscopy to InvestigateIntracellular Trafficking of Toll-Like Receptors.- Assessing theInhibitory Activity of Oligonucleotides on TLR7 Sensing.- Methods forDelivering DNA to Intracellular Receptors.- Detection of Interactionbetween Toll-Like Receptors and other Transmembrane Proteins byCo-Immunoprecipitation Assay.- Flow Cytometry-Based Bead-Binding Assay forMeasuring Receptor Ligand Specificity.- Measuring Monomer-to-FilamentTransition of MAVS as an In Vitro Activity Assay for RIG-I-Like Receptors.- Co-TranscriptomicAnalysis by RNA Sequencing to Simultaneously Measure Regulated Gene Expressionin Host and Bacterial Pathogen.- Simple Methods to Investigate MicroRNAInduction in Response to Toll-Like Receptors.- Determining the Function ofLong Noncoding RNA in Innate Immunity.- Analysis of Post-TranscriptionalGene Regulation of Nod-Like Receptors via the 3'UTR.- TLR Function inMurine CD4⁺ T Lymphocytes and their Role in Inflammation.- FlowCytometry Analysis of B Cell Activation and Antibody Responses Induced byToll-Like Receptors.- Toll-Like Receptor-Dependent Immune Complex Activation ofB Cells and Dendritic Cells.- Analysis of TLR-Induced Metabolic Changes inDendritic Cells Using the Seahorse XF^e96 Extracellular FluxAnalyzer.- Toll-Like Receptor Signaling and the Control of IntestinalBarrier Function.- Understanding the Role of Cellular Molecular Clocks inControlling the Innate Immune Response.- Methods to Investigate the Roleof Toll-Like Receptors in Allergic Contact Dermatitis.- Allergens andActivation of the Toll-Like Receptor Response.- Investigating the Role ofToll-Like Receptorsin Models of Arthritis.- Delineating the Role ofToll-Like Receptors in the Neuro-Inflammation Model EAE.- The Use of MiRNAAntagonists in the Alleviation of Inflammatory Disorders.- Investigatingthe Role of Toll-Like Receptors in Mouse Models of Gastric Cancer.

Summary

This second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction with other cellular and biologic systems, and cancer progression. This book is divided into five sections: Part I outlines methods for TLR detection, interaction, and intracellular trafficking; Part II describes methods and assays to investigate how TLRs cross-prime other pattern recognition receptors, including intracellular DNA receptors and inflammasome formation; Part III highlights RNA regulation, detailing how TLRs can induce RNA transcripts and molecules such as lncRNAs and microRNAs; Part IV explores TLR detection and activation in systems such as epithelial barrier function, metabolism and the circadian clock, as well as cellular systems including T and B lymphocytes; and Part V describes models to delineate the role of TLRs in diseases such as dermatitis, arthritis, and gastric cancer. Written in the highly successful Methods in Molecular Biology series format, each chapter contains a summary, a list of required materials, step-by-step, readily reproducible laboratory protocols, useful notes to investigate TLRs in cell culture, systems and disease, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Toll-Like Receptors: Methods and Protocols, Second Edition is a valuable resource to any immunologist, molecular or medical biologist working in a laboratory setting. It will add skill to both students and the more advanced molecular biologist who wishes to learn a new technique or move to a different area within their current repertoire of practical knowledge.

Product details

• Assisted by: Clair McCoy (Editor), Claire McCoy (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 01.01.2018
• EAN: 9781493980239
• ISBN: 978-1-4939-8023-9
• No. of pages: 455
• Dimensions: 180 mm x 24 mm x 253 mm
• Weight: 891 g
• Illustrations: XIV, 455 p. 58 illus., 29 illus. in color.
• Series: Methods in Molecular Biology; Methods in Molecular Biology
• Subjects: Natural sciences, medicine, IT, technology > Medicine > Clinical medicine; B, Immunology, Life Sciences, biochemistry, proteins, Biomedical and Life Sciences, Receptors