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This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.
List of contents
Multi-Parametric Screening in Arabidopsis thaliana Seedlings.- Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress.- Method for Ultra-Rapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen.- A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death using Suspension Cell Culture of Arabidopsis thaliana.- High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression.- Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana using Chemical Genetics.- Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls using BY-2 Cells.- Small Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes.- High-Throughput In Vitro Screeningfor Inhibitors of Cereal -glucosidase.- Chemical Screening for Strigolactone Receptor Antagonists using Arabidopsis thaliana.- Chemical Control of ABA Receptors to Enable Plant Protection against Water Stress.- Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening.- Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside that Controls the Leaf Closure of Samanea saman.- Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule.- Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants using PlantiSMASH.- Chemical Genomics Translatability from Unicellular to Multicellular Models.- Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii.- FnCpf1-Mediated Targeted Mutagenesis in Plants.
Summary
This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.
