Single-Molecule Studies of Nucleic Acids and Their Proteins

This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.

List of contents

• 1: Introduction to DNA


• 2: Manipulating DNA


• 3: Fluorescence Spectroscopy and Microscopy Techniques


• 4: The Mechanical Properties of Nucleic Acids


• 5: Structural Transitions in DNA


• 6: Single Molecule Studies of DNA-associated Motors


• 7: DNA and RNA Polymerases


• 8: DNA Helicases


• 9: Topoisomerases


• 10: Conclusions

About the author

David Bensimon and Vincent Croquette have been co-leaders of a group at the LPS-ENS that has been studying single DNA molecules and their interactions with proteins since 1991. Jean-François Allemand, Terence Strick and Xavier Michalet have been their students and collaborators ever since. They have jointly developed magnetic traps and have been studying various aspects of single molecule DNA/protein transactions. Since joining the lab of Professor S. Weiss at UCLA , Xavier Michalet has been focusing on the use of fluorescent and spectroscopic methods to study single biomolecules.

Summary

This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.

Report

this book is helpful for different audiences... The volume is very well written and enjoyable to read. Robert Ros, The Quarterly Review of Biology

Product details

• Authors: Jean-francois Allemand, Jean-François Allemand, Jean-Francois (Professor Allemand, David Bensimon, David (Research Director and Professor Bensimon, Vincent Croquette, Vincent (Research Director Croquette, Xavier Michalet, Xavier (Researcher Michalet, Terence Strick, Terence (Research Director and Principle Investigator Strick, Strick Terence
• Publisher: Oxford University Press
• Languages: English
• Product format: Hardback
• Released: 28.06.2018
• EAN: 9780198530923
• ISBN: 978-0-19-853092-3
• No. of pages: 240
• Series: Oxford Graduate Texts; Oxford Graduate Texts
• Subjects: Natural sciences, medicine, IT, technology > Physics, astronomy > Atomic physics, nuclear physics; ORGANIC CHEMISTRY, SCIENCE / Life Sciences / Biochemistry, SCIENCE / Physics / Atomic & Molecular, SCIENCE / Physics / Condensed Matter, SCIENCE / Life Sciences / Molecular Biology, biochemistry, molecular biology, proteins, Biophysics, Atomic & molecular physics, Enzymology, Atomic and Molecular Physics, Mesoscopic Physics