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Marine enzymes and specialized metabolism - Part B, Volume 605 in the Methods in Enzymology series, highlights experimental methods on diverse marine enzymes involved in the construction of bioactive natural product molecules. Unique sections in this new release include discussions on polysaccharide-degrading enzymes from marine gastropods, radical SAM epimerases from sponge microbes, DMS/P demethylase in bacteria, reconstitution of particulate methane monooxygenase into membrane mimetics, the structure and function of cyanobactin enzymes, marine cyanobacterial polyketide beta-branching enzymology, marine cyanobacterial PKS-NRPS enzymology and structural biology, biochemical profiling of DMSP lyases, and more.
List of contents
1. Polysaccharide-degrading enzymes from marine gastropods
Takao Ojima
2. Radical SAM epimerases from sponge microbes
Joern Piel
3. DMS/P demethylase in bacteria
Chris Reisch
4. Reconstitution of particulate methane monooxygenase into membrane mimetics
Amy C. Rosenzweig
5. Structure and function of cyanobactin enzymes
Eric W. Schmidt
6. Marine cyanobacterial polyketide beta-branching enzymology
David Sherman
7. Marine cyanobacterial PKS-NRPS enzymology and structural biology
Janet Smith
8. Biochemical profiling of DMSP lyases
Dan Tawfik
9. Preparation and characterization of the enterocin favorskiiase flavoprotein EncM and its distinctive flavin-N5-oxide cofactor
Robin Teufel
10. Unusual "head-to-torso"-coupling of terpene precursors as a new strategy for the structural diversification of natural products
Robin Teufel
11. Soluble proton ATPases from marine organisms
Martin Tresguerres
12. Prochlorosin enzymology in marine cyanobacteria
Wilfred van der Donk
13. The Biosynthetic Insights on MIsakinolide and Calyculin in Candidatus 'Entotheonella'
Toshiyuki Wakimoto
14. Algal vanadium-dependent haloperoxidases
Ron Wever
15. Methane biosynthesis and phosphonates in pelagic bacteria
David Zechel
16. Terminal alkyne biosynthesis in marine microbes
Wenjun Zhang
About the author
Bradley S. Moore is a Professor at the Scripps Institution of Oceanography, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego.
