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This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune systems have contributed the chapters that aim to guide readers through these complex methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Intravital Imaging of Dynamic Bone and Immune Systems: Methods and Protocols will serve as an idea guide for general readers in order to establish and perform these experiments by their own.
List of contents
Bone Imaging: Osteoclast and Osteoblast Dynamics.- Intravital Imaging of Mouse Bone Marrow: Hemodynamics and Vascular Permeability.- Bone Imaging: Platelet Formation Dynamics.- Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices.- Two-Photon Imaging of T-Cell Motility in Lymph Nodes: In Vivo and Ex Vivo Approaches.- Imaging the Lymph Node Stroma.- Intravital Imaging of B Cell Responses in Lymph Nodes.- Live Imaging of the Skin Immune Responses: Visualization of the Contact Hypersensitivity Response.- Imaging of Inflammatory Responses in the Mouse Ear Skin.- In Vivo Imaging of Immune Cells in Peyer's Patches.- Intravital Imaging of T Cells Within the Spinal Cord.- Kidney Imaging: Intravital Microscopy.- Intravital Imaging of Liver Cell Dynamics.- Intravital Imaging of the Heart at the Cellular Level Using Two-Photon Microscopy.- Imaging Window Device for Subcutaneous Implantation Tumor.- New Tools for Imaging of Immune Systems: Visualization of Cell Cycle, Cell Death,and Cell Movement by Using the Mice Lines Expressing Fucci, SCAT3.1, and Kaede and KikGR.
Summary
This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune systems have contributed the chapters that aim to guide readers through these complex methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Intravital Imaging of Dynamic Bone and Immune Systems: Methods and Protocols will serve as an idea guide for general readers in order to establish and perform these experiments by their own.
