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This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental methods for quantification of different root developmental processes to complex methods that require sophisticated equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Root Development: Methods and Protocols serves as a valuable tool for plant biologists specialized in root development, from beginners to experienced researchers in the field.
List of contents
Measuring Plant Root Traits Under Controlled and Field Conditions: Step-By-Step Procedures.- Phenotyping Crop Root Crowns: General Guidance and Specific Protocols for Maize, Wheat, and Soybean.- Developmental Analysis of Arabidopsis Root Meristem.- Genetic and Phenotypic Analysis of Lateral Root Development in Arabidopsis thaliana.- Adapting the Lateral Root Inducible System to Medicago truncatula.- Characterization of Root Epidermal Cell Patterning and differentiation in Arabidopsis.- In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls.- Root Gravitropism: Quantification, Challenges, and Solutions.- Calcium Ion Dynamics in Roots: Imaging and Analysis.- Optimized Whole-Mount In Situ Immunolocalization for Arabidopsis thaliana Root Meristems and Lateral Root Primordia.- Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development.- Histological Profiling Over Time to Optimize Root Cell Type-Specific Reporter Lines for Cell Sorting.- Long-Term In Vivo Imaging of Luciferase-Based Reporter Gene Expression in Arabidopsis Roots.- Cortical Cell Length Analysis During Gravitropic Root Growth.- Growth Rate Normalization Method to Assess Gravitropic Root Growth.- Immunoprecipitation of Membrane Proteins from Arabidopsis thaliana Root Tissue.- Monitoring Transcriptomic Changes in Soil-Grown Roots and Shoots of Arabidopsis thaliana Subjected to a Progressive Drought Stress.- Chromatin Immunoprecipitation Sequencing (ChIP-Seq) for Transcription Factors and Chromatin Factors in Arabidopsis thaliana Roots: From Material Collection to Data Analysis.- miChIP-Seq for Genome-Wide Mapping of In Vivo TF-DNA Interactions in Arabidopsis Root Protoplasts.- Proteome Analysis of Arabidopsis Roots.- Step-By-Step Construction of Gene Co-Expression Networks from High-Throughput Arabidopsis RNA Sequencing Data.- GWA-Portal: Genome-Wide Association Studies Made Easy.
Summary
This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental methods for quantification of different root developmental processes to complex methods that require sophisticated equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Root Development: Methods and Protocols serves as a valuable tool for plant biologists specialized in root development, from beginners to experienced researchers in the field.
