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This volume explores protocols for identifying mutant mice and characterizing parts of their anatomical, functional, cellular, and molecular phenotypes. The chapters in this book look at anatomical and functional phenotyping using quantitative imaging, isolation of specific embryonic cell types for cell culture, analysis of gene expression, and how to define chromatin structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Mouse Embryogenesis: Methods and Protocols is a valuable resource for experimentalists interested in discovering new aspects of embryogenesis control, organ function, and the origin of disease.
List of contents
Mouse Genotyping.- Visualizing the Vascular Network in the Mouse Embryo and Yolk Sac.- In Vivo Evaluation of the Cardiovascular System of Mouse Embryo and Fetus Using High Frequency Ultrasound.- Dynamic Imaging of Mouse Embryos and Cardiodynamics in Static Culture.- In Vivo Imaging of the Mouse Reproductive Organs, Embryo Transfer, and Oviduct Cilia Dynamics Using Optical Coherence Tomography.- Live Imaging of Fetal Intra-Abdominal Organs Using Two-Photon Laser-Scanning Microscopy.- Embrionary Mouse Cardiac Fibroblast Isolation.- Explant Culture for Studying Lung Development.- Isolating Embryonic Cardiac Progenitors and Cardiac Myocytes by Fluorescence-Activated Cell Sorting.- Isolation and Culture of Mouse Placental Endothelial Cells.- Flow Cytometry and Lineage Tracing Study for Identification of Adipocyte Precursor Cell (APCs) Populations.- Whole-Mount and Section In Situ Hybridization in Mouse Embryos for Detecting mRNA Expression and Localization.- Chromosome Painting of Mouse Chromosomes.- Chromatin Immunoprecipitation in Early Mouse Embryos.- Shaping Up the Embryo: The Role of Genome 3D Organization.- Genome Edition during Development Using the CRISPR-Cas Technology.
Summary
This volume explores protocols for identifying mutant mice and characterizing parts of their anatomical, functional, cellular, and molecular phenotypes. The chapters in this book look at anatomical and functional phenotyping using quantitative imaging, isolation of specific embryonic cell types for cell culture, analysis of gene expression, and how to define chromatin structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Mouse Embryogenesis: Methods and Protocols is a valuable resource for experimentalists interested in discovering new aspects of embryogenesis control, organ function, and the origin of disease.
