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This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries.
List of contents
Heterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level.- Integrating Analysis of Cellular Heterogeneity in High Content Dose-Response Studies.- Image-Based Tracking of Heterogeneous Single Cell Phenotypes.- High-Dimensional Single Cell Analysis with Mass Cytometry.- Spectral and Imaging Flow Cytometry in Phytoplankton Research.- X-Ray Fluorescence Detected Flow Cytometry.- Multiparametric Analysis of Myeloid Populations by Flow Cytometry.- Quantitation of IRF3 Nuclear Translocation in Heterogeneous Cellular Populations from Cervical Tissue Using Imaging Flow Cytometry.- Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry.- Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System during Neuroinflammation and Neurodegeneration.- Analysis of Microtubule Dynamics Heterogeneity in Cell Culture.- Heterogeneity of Focal Adhesions and Focal Contacts in Motile Fibroblasts.- Laser Tweezers Raman Micro-Spectroscopy of Single Cells and Biological Particles.- Quantification of the Metabolic Heterogeneity in Mycobacterial Cells Through the Measurement of the NADH/NAD+ Ratio Using a Genetically-Encoded Sensor.- Characterizing Cell Heterogeneity Using PCR Fingerprinting of Surface Multigene Families in Protozoan Parasites.- Assessing Carbon Source-Dependent Phenotypic Variability in Pseudomonas putida.- The Retinal Pigment Epithelial Cell Line (ARPE-19) Displays Mosaic Structural Chromosomal Aberrations.- FACS Isolation of Viable Cells in Different Cell Cycle Stages from Asynchronous Culture for RNA-Sequencing.- Measuring Nanoscale Chromatin Heterogeneity with Partial Wave Spectroscopic Microscopy.
Summary
This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries.
