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The objective of this practical handbook is to provide ecologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practice studies and analyses based on environmental DNA.
List of contents
- 1: Introduction to environmental DNA (eDNA)
- 2: DNA metabarcode choice and design
- 3: Reference databases
- 4: Sampling
- 5: DNA extraction
- 6: DNA amplification and multiplexing
- 7: DNA sequencing
- 8: DNA metabarcoding data analysis
- 9: Single-species detection
- 10: Environmental DNA for functional diversity
- 11: Some early landmark studies
- 12: Freshwater ecosystems
- 13: Marine environments
- 14: Terrestrial ecosystems
- 15: Palaeoenvironments
- 16: Host-associated microbiota
- 17: Diet analysis
- 18: Analysis of bulk samples
- 19: The future of eDNA metabarcoding
About the author
After teaching biology in high school for 10 years, Pierre Taberlet obtained his PhD in ecology in 1992 at the Université Grenoble Alpes. He is currently senior CNRS scientist in Laboratoire d'Ecologie Alpine at the Université Grenoble Alpes. He has been heavily involved in developing innovative molecular tools for tackling ecological questions. He was the recipient of the Molecular Ecology Prize in 2007 and is a member of the Norwegian Academy of Sciences and Letters (2005) and of Academia Europaea (2014).
Aurélie Bonin obtained her PhD in genomics of local adaption in 2006. After various postdoctoral positions in population genomics and ecology, she is now a DNA metabarcoding project leader for the French AnaEE (Analysis and Experimentation on Ecosystems).
Lucie Zinger obtained her PhD in microbial ecology in 2009. She is currently assistant professor at the Institut de Biologie de l'École Normale Supérieure in Paris. She is interested in the fields of biogeography, macroecology, community ecology, and microbial ecology, as well as eDNA-based techniques to uncover the diversity of elusive organisms. Her current work focuses on the distribution and assembly mechanisms of microorganisms in soils, oceans, plant leaves, and insects.
Geneticist and bioinformatician, Eric Coissac obtained his PhD in genetics and genomics in 1996 at the Université Pierre et Marie Curie in Paris. He is currently associate professor at Université Grenoble Alpes in Laboratoire d'Écologie Alpine.
Summary
The objective of this practical handbook is to provide ecologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practice studies and analyses based on environmental DNA.
Additional text
In a world faced with accelerating environmental change and loss of biodiversity, this book is a timely overview of eDNA as a complementary and noninvasive approach for investigating and monitoring biodiversity ... an ideal introduction to all ecologists looking to eDNA as a method of choice, but also speaks to the more experienced researchers in molecular ecology. Lastly, it provides textbook material for university courses around the world, where eDNA is continuously increasing in popularity.
