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This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for studies of RNA-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Drosophila Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
List of contents
Drosophila melanogaster Oogenesis: An Overview.- Basic Techniques in Drosophila Ovary Preparation.- Mosaic Analysis in the Drosophila Melanogaster Ovary.- Genetic Mosaic Analysis of Stem Cell Lineages in the Drosophila Ovary.- Culturing Drosophila Egg Chambers and Investigating Developmental Processes through Live Imaging.- Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement.- Visualizing Microtubule Networks during Drosophila Oogenesis using Fixed and Live ImagingVisualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers.- Single Molecule RNA in situ Hybridization (smFISH) and Immunofluorescence (IF) in the Drosophila Egg Chamber.- Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries.- Ultrastructural Analysis of Drosophila Ovaries by Electron Microscopy.- Immuno-Electron Microscopy and Electron Microscopic in situ Hybridization for Visualizing piRNA Biogenesis Bodies in Drosophila Ovaries.- Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry.-Detection of Cell Death and Phagocytosis in the Drosophila Ovary.- Analysis of Cell Cycle Switches in Drosophila Oogenesis.- Global Run-On Sequencing (GRO-seq) Library Preparation from Drosophila Ovaries.- Bioinformatics Analysis to Identify RNA-protein Interactions in Oogenesis.
Summary
This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for studies of RNA-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Drosophila Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
