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This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come.
List of contents
A Historical Perspective on Bacterial Persistence.- Persisters-Methods for Isolation and Identifying Contributing Factors: A Review.- A General Method for Measuring Persister Levels in Escherichia Coli Cultures.- Optimized Method for Measuring Persistence in Escherichia cCli with Improved Reproducibility.- A Micro plate-Based System as In Vitro Model of Biofilm Growth and Quantification.- Protocol for Determination of the Persister Subpopulation in Candida Albicans Biofilms.- Quantitative Measurements of Type I and Type II Persisters Using ScanLag.- Analyzing Persister Physiology with Fluorescence-Activated Cell Sorting.- Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array.- A Whole-Cell Based High-Throughput Screening Method to Identify Molecules Targeting Pseudomonas Aeruginosa Persister Cells.- Functional Analysis of the Role of Toxin-Antitoxin (TA) Loci in Bacterial Persistence.- Experimental Evolution of Escherichia Coli Persister Levels Using Cyclic Antibiotic Treatments.- In Vitro Models for the Study of the Intracellular Activity of Antibiotics.- A Murine Model for Escherichia Coli Urinary Tract Infection.- Analysis of Macrophage-Induced Salmonella Persisters.- Population Dynamics Analysis of Ciprofloxacin-Persistent S. Typhimurium Cells in a Mouse Model for Salmonella Diarrhea.- Computational Methods to Model Persistence.
About the author
Jan Michiels Centre of Microbial and Plant Genetics Department of Microbial and Molecular Systems, KU Leuven - University of Leuven Kasteelpark Arenberg 20 box 2460, B-3001 Heverlee Phone: +32 16 32 16 31 (secretariat) or +32 16 32 96 84 (office) Fax.: +32 16 32 19 63 Email: jan.michiels@biw.kuleuven.be Maarten Fauvart Centre of Microbial and Plant Genetics Department of Microbial and Molecular Systems, KU Leuven - University of Leuven Kasteelpark Arenberg 20 box 2460, B-3001 Heverlee Tel: +32 16 32 16 31 (secretariat) or +32 16 32 96 80 (office); Fax: +32 16 32 19 63 Email: maarten.fauvart@biw.kuleuven.be
Summary
This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come.
