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List of contents
The Emerging Field of RNA Nanotechnology. Uniqueness, Advantages, Challenges, Solutions, and Perspectives in Therapeutics Applying RNA Nanotechnology. Kink Turn Structural Motif in RNA. RNA Nanotechnology: Learning from Biologically Active RNA Nanomachines. Natural Selection and Structural Polymorphism of RNA 3D Structures Involving GNRA Loops and Their Receptor Motifs . RNA Junction Motifs as Scaffolds for Construction of Multifunctional RNA Nanoparticles. Role of Dynamics in RNA Nanostructure Design. RNA Three-Dimensional Structure Determination Using Experimental Constraints. Nucleotide Chemistry for RNA Nanoparticle Labeling, Conjugation, and Synthesis. RNA Conjugations and Ligations for RNA Nanotechnology. Atom-Specific Mutagenesis of RNAs for Structure, Function, and Therapeutics Studies. Atomic Force Microscopy of RNA: Imaging and Beyond. Single-Molecule Approach to Study RNA Nanoparticles. Fabrication Methods for RNA Nanoparticle Assembly Based on Bacteriophage phi29 Packaging RNA Structural Features. Synthetic RNA–Protein Nanostructures and Their Potential Applications. DNA Nanotechnology as Reference for RNA Nanotechnology. Thermodynamically Stable RNA Three-Way Junction for Constructing Multifunctional Nanoparticles for Delivery of Therapeutics. Design and Construction of RNA Nanoparticles Targeting Prostate Cancer. Conjugation of RNA Aptamer to RNA Nanoparticles for Targeted Drug Delivery. Extracellular Membrane Vesicles and Extracellular Membrane Vesicle–Based Therapeutics for Brain Diseases. Pharmacokinetics and Pharmacodynamics of RNA Nanoparticles. RNA Nanotechnology in Sensing, Detection, and Disease Diagnosis. Potentials of RNA Aptamers for Viral Detection and Treatment. Application of RNA Aptamers in Nanotechnology and Therapeutics . Aptamers Targeting a Subunit or a Conformation of Glutamate Ion Channel Receptors. Application of MicroRNAs in RNA Nanotechnology and Antiviral Therapeutics. MicroRNAs: Biology and Role in RNA Nanotechnology. Current Advances in Self-Assembly RNAi Nanoparticles. Self-Assembly of siRNA Containing Nanoparticles. Index.
About the author
Peixuan Guo is the Director of the NIH Nanomedicine Development Center at the University of Cincinnati, Ohio and the Director of the NIH/NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy at Purdue University, Indiana.
Summary
Interest in RNA nanotechnology has increased in recent years as recognition of its potential for applications in nanomedicine has grown. Edited by the world's foremost experts in nanomedicine, this comprehensive, state-of-the-art reference details the latest research developments and challenges in the biophysical and single molecule approaches in RNA nanotechnology. In addition, the text also provides in-depth discussions of RNA structure for nanoparticle construction, RNA computation and modeling, single molecule imaging of RNA, RNA nanoparticle assembly, RNA nanoparticles in therapeutics, RNA chemistry for nanoparticle synthesis, and conjugation and labeling.
Additional text
"The main strength of this book is in its focus on RNA as a functional agent. RNA can be derivatized using a variety of biochemical technologies to incorporate therapeutic or imaging functionalities. This book spans different aspects of techniques that are necessary to obtain functional RNA nanoparticles, including computational design, synthesis of components, assembly of functional units, and their biochemical and biophysical characterization. It also discusses promises and challenges in the applications of RNA nanoparticles as therapeutics. … This book is unique in that it covers a very broad spectrum of topics related to RNA nanotechnology, yet it maintains a focus on constructing and analyzing RNA nanoparticles. It introduces the reader to the basics of RNA structure, and discusses functions of various natural RNAs, from small molecules to large molecular machines such as the ribosome. It also introduces the concept of designing new RNAs, and provides a comprehensive coverage of modern techniques, linking theory and experiment. Technologies discussed include RNA structure prediction, prediction of interactions, simulations of RNA folding, computational design, chemical methods for RNA nanoparticle synthesis, assembly, and labeling, as well as methods for their biophysical and biochemical characterization and biomolecular imaging. … Chapters 1, 4, 7 are very exciting. Chapter 1 is a condensed introduction to the topic of RNA nanotechnology and to challenges and opportunities in using the RNA molecules as therapeutic agents. It is quite obvious that Dr. Guo is an enthusiast of RNA nanotechnology and in fact, this enthusiasm is quite contagious. Chapters 4 and 7 provide more focused description of two issues: 1) naturally existing RNAs as molecular machines, with a very informative discussion of differences between molecular machines and macro-machines, using ribosome as an example and 2) the role of dynamics (as opposed to 3D structure alone) in RNA design. Both these chapters demonstrate the extraordinary interdisciplinary character of the field of RNA nanotechnology, as well asof this very book. I definitely want to have this book on my shelf, and I intend to read all chapters as soon as I get it!"––Prof. Janusz M. Bujnicki, International Institute of Molecular and Cell Biology in Warsaw, and Adam Mickiewicz University in Poznań, Poland
"This book is very good in this field. I want to have this book on my shelf. … This book has a broad coverage from principles and fundamentals of RNA nanotechnology, RNA nanotechnology design and construction to diagnostic and therapeutic application. These detailing descriptions provide a clear and complete picture of up-to-date knowledge of RNA nanotechnology and applications." ––Prof. Shu-Feng Zhou, University of South Florida
"The range of topics is excellent. … A nice collection of, basic and focused, articles on RNA."nanotechnology, a fast developing field."—Eric Westhof, IBMC-CNRS, Université de Strasbourg, France
