Single-Molecule Fluorescence Spectroscopy of the Folding of a Repeat Protein

Inthis thesis single-molecule fluorescence resonance energy transfer(FRET)spectroscopy was used to study the folding of a protein that belongsto the large and important family of repeat proteins. Cohen showsthat the dynamics of the expanded conformations is likely to be veryfast, suggesting a spring-like motion of the whole chain. Thefindings shed new lighton the elasticity of structure in repeat proteins, which is relatedto their function in binding multiple and disparate partners. Thisconcise research summary provides useful insights for studentsbeginning a PhD in this or a related area, and researchers enteringthis field.

List of contents

Abstract.- Introduction.- Methods.- Results.- Discussion.- Summary and Future Plans.

About the author

Sharona Cohen is a trained physicist, specialized in optical systems, microscopy and spectroscopy. She acquired her PhD at the interdisciplinary chemical physics department at Weizmann Institute of Science.

Summary

In
this thesis single-molecule fluorescence resonance energy transfer
(FRET)
spectroscopy was used to study the folding of a protein that belongs
to the large and important family of repeat proteins. Cohen shows
that the dynamics of the expanded conformations is likely to be very
fast, suggesting a spring-like motion of the whole chain. The
findings shed new light
on the elasticity of structure in repeat proteins, which is related
to their function in binding multiple and disparate partners. This
concise research summary provides useful insights for students
beginning a PhD in this or a related area, and researchers entering
this field.

Product details

• Authors: Sharona Cohen
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 01.01.2016
• EAN: 9783319372365
• ISBN: 978-3-31-937236-5
• No. of pages: 59
• Dimensions: 155 mm x 4 mm x 235 mm
• Weight: 134 g
• Illustrations: XIII, 59 p.
• Series: Springer Theses; Springer Theses
• Subjects: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics; Biophysik, Biochemie, B, Spektroskopie, Spektrochemie, Massenspektrometrie, Chemistry and Materials Science, spectroscopy, Spectrum analysis, spectrochemistry, mass spectrometry, Spectroscopy/Spectrometry, Medical physics, Biophysics, Biological physics, Biological and Medical Physics, Biophysics, Bioorganic Chemistry