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This detailed volume explores the most popular antigen production and delivery strategies that have been tested in veterinary species. Viral vectors as well as genetic and protein subunit vaccines or large scale protein production systems are considered as well as an updated view of most options available for vaccine development, including the data obtained through experimental trials which contributes to the exploration and understanding of the immune mechanisms and immune correlates relevant in protection among different animal species. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols facilitates access to well-established protocols to those beginning in this interesting and laborious field as well as providing important basic knowledge when attempting a novel vaccine design or platform.
List of contents
Vaccines and Vaccination for Veterinary Viral Diseases: A General Overview.- Using IC-Tagging Methodology for Production and Purification of Epitope-Loaded Protein Microspheres for Vaccination.- Plant-Based Vaccine Antigen Production.- DNA Vaccines: Experiences in the Swine Model.- Novel Adjuvants and Immunomodulators for Veterinary Vaccines.- Polymerase Mechanism-Based Method of Viral Attenuation.- BacMam Platform for Vaccine Antigen Delivery.- Laboratory-Scale Production of Replication-Deficient Adenovirus Vectored Vaccines.- Generation of Recombinant Modified Vaccinia Virus Ankara Encoding VP2, NS1, and VP7 Proteins of Bluetongue Virus.- Generation of Recombinant Capripoxvirus Vectors for Vaccines and Gene Knockout Function Studies.- Recombinant Swinepox Virus for Veterinary Vaccine Development.- Generation and Selection of Orf Virus (ORFV) Recombinants.- Polycistronic Herpesvirus Amplicon Vectors for Veterinary Vaccine Development.- Construction and Application of Newcastle Disease Virus-Based Vector Vaccines.- Chimeric Pestivirus Experimental Vaccines.- Analysis of the Cellular Immune Responses to Vaccines.
Summary
This detailed volume explores the most popular antigen production and delivery strategies that have been tested in veterinary species. Viral vectors as well as genetic and protein subunit vaccines or large scale protein production systems are considered as well as an updated view of most options available for vaccine development, including the data obtained through experimental trials which contributes to the exploration and understanding of the immune mechanisms and immune correlates relevant in protection among different animal species. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols facilitates access to well-established protocols to those beginning in this interesting and laborious field as well as providing important basic knowledge when attempting a novel vaccine design or platform.
