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Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Live Cell Imaging: Methods and Protocols promises to contribute greatly to the further development and dissemination of this fundamentally important technology which spans across many disciplines including molecular and cell biology, chemistry, physics, optics, engineering, cell physiology, and medicine.
List of contents
I: General Principles and Overview.- Instrumentation for Live-Cell Imaging and Main Formats.- Labels and Probes for Live Cell Imaging: Overview and Selection Guide.- Live Cell Imaging: An Industrial Perspective.- II: Imaging Techniques, Probes, and Applications.- Design of Fluorescent Fusion Protein Probes.- Synthetic Fluorescent Probes for Imaging of Peroxynitrite and Hypochlorous Acid in Living Cells.- Photo-Activatable Probes for the Analysis of Receptor Function in Living Cells.- The Application of Fluorescent Probes for the Analysis of Lipid Dynamics During Phagocytosis.- Imaging of Mitotic Cell Division and Apoptotic Intra-Nuclear Processes in Multicolor.- Manipulation of Neutrophil-Like HL-60 Cells for the Study of Directed Cell Migration.- A Method for Analyzing Protein-Protein Interactions in the Plasma Membrane of Live B Cells by Fluorescence Resonance Energy Transfer Imaging as Acquired by Total Internal Reflection Fluorescence Microscopy.- Sample Preparation for STED Microscopy.- Two-Photon Permeabilization and Calcium Measurements in Cellular Organelles.- Imaging and Analysis of Three-Dimensional Cell Culture Models.- Long-Term Imaging in Microfluidic Devices.- Monitoring of Cellular Responses to Hypoxia.- Imaging of Cellular Oxygen and Analysis of Metabolic Responses of Mammalian Cells.- Analysis of Mitochondrial pH and Ion Concentrations.- Live Cell Imaging Analysis of Receptor Function.- Subcellular Dynamic Imaging of Protein-Protein Interactions in Live Cells by Bioluminescence Resonance Energy Transfer.- Quantitative Analysis of Membrane Potentials.- Image Correlation Spectroscopy to Define Membrane Dynamics.
Summary
Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Live Cell Imaging: Methods and Protocols promises to contribute greatly to the further development and dissemination of this fundamentally important technology which spans across many disciplines including molecular and cell biology, chemistry, physics, optics, engineering, cell physiology, and medicine.
